Neuroninių grandinių dekodavimas, kontroliuojančios kompulsinį sacharozę (2015) (BINGE MECHANISM)

COMMENTS: A study that isolates the circuit responsible for compulsive sugar consumption. This circuit is separate from the normal food consumption circuit, and seems to be the “binge mechanism”. Does compulsive sexual behavior has its own circuit?


Edward H. Nieh2 Gillian A. Matthews2 Stephen A. Allsop2 Kara N. Presbrey Christopher A. Leppla Romy Wichmann Rachael Neve Craig P. Wildes Kay M. Tyekorespondencija 2Co-first autorius

Pabrėžia

  • • LH-VTA neuronai koduoja atlygio ieškojimo veiksmus po to, kai jie pereina į įpročius
  • • LH neuronų pogrupis pasroviui VTA koduoja atlygio lūkesčius
  • • LH-VTA projekcijos užtikrina dvipusę kompulsinio sacharozės paieškos kontrolę
  • • LH-VTA aktyvinimas GABAerginės projekcijos didina netinkamą gniwing elgesį

Santrauka

Šoninė hipotalamio (LH) projekcija į ventralinę tegmentalinę sritį (VTA) buvo susieta su atlygio apdorojimu, tačiau LH-VTA kilpos skaičiavimus, kurie sukelia specifinius elgesio aspektus, buvo sunku atskirti. Mes parodome, kad LH-VTA neuronai koduoja išmoktą atlygio paieškos veiksmą, nepriklausantį nuo atlygio prieinamumo. Priešingai, LH neuronai, esantys pasroviui nuo VTA, koduoja atlygio prognozavimo ženklus ir netikėtą atlygio praleidimą. Mes parodome, kad LH-VTA kelio slopinimas mažina „kompulsinį“ sacharozės ieškojimą, bet ne maisto vartojimą alkanas pelėms. Mes atskleidžiame, kad LH siunčia VIT dopamino (DA) ir GABA neuronų sužadinimo ir slopinimo įvestį ir kad GABAerginė projekcija skatina su maitinimu susijusį elgesį. Mūsų tyrimas apima informaciją apie LH neuronų tipą, funkciją ir ryšį ir nurodo neuroninę grandinę, kuri selektyviai kontroliuoja kompulsinį cukraus vartojimą, užkertant kelią maitinimui, kuris būtinas išgyvenimui, suteikiant galimą tikslą gydomoms intervencijoms dėl kompulsinio sutrikimo.

Įvadas

Tremendous heterogeneity exists across lateral hypothalamic (LH) neurons in terms of function and connectivity, and this can be observed by the variety of behaviors related to reward, motivation, and feeding linked with this region. However, little is known about how the LH computes specific aspects of reward processing and how this information is relayed to downstream targets. Electrical stimulation of the LH produces intracranial self-stimulation (ICSS) (Olds and Milner, 1954), as well as grooming, sexual, and gnawing behaviors (Singh et al., 1996). LH neurons encode sensory stimuli (Norgren, 1970, Yamamoto et al., 1989), including reward-associated cues (Nakamura et al., 1987). LH neurons also fire during both feeding (Burton et al., 1976, Schwartzbaum, 1988) and drinking (Tabuchi et al., 2002). However, making sense of the remarkable functional heterogeneity observed in the LH has been a major challenge in the field.

Although the LH is interconnected with many subcortical regions, we have a poor understanding of how the functional and cellular heterogeneity of the LH is transposed upon these anatomical connections. One LH projection target of interest is the ventral tegmental area (VTA), a critical component in reward processing (Wise, 2004). The LH-VTA projection was explored in early studies that used electrophysiological recordings combined with antidromic stimulation (Bielajew and Shizgal, 1986, Gratton and Wise, 1988). It has since been confirmed, using a rabies-virus-mediated tracing approach, that there is monosynaptic input from LH neurons onto dopamine (DA) neurons in the VTA (Watabe-Uchida et al., 2012). The VTA also sends reciprocal projections back to the LH, both directly and indirectly via other regions such as the nucleus accumbens, amygdala, hippocampus, and ventral pallidum (Barone et al., 1981, Beckstead et al., 1979, Simon et al., 1979).

Although both electrical (Bielajew and Shizgal, 1986) and optical (Kempadoo et al., 2013) stimulation have established a causal role for the LH projection to the VTA in ICSS, several questions remain to be answered. First, what is the neural response of LH-VTA neurons to different aspects of reward-related behaviors? Second, what is the role of the LH-VTA projection in reward seeking under different reinforcement contingencies? Third, what is the overall composition of fast transmission mediated by LH inputs to the VTA, and which VTA cells receive excitatory/inhibitory input? Finally, what do the excitatory and inhibitory components of the LH-VTA pathway each contribute toward orchestrating the pursuit of appetitive reward?

To address these questions, we recorded from LH neurons in freely moving mice and used optogenetic-mediated photoidentification to overlay information about the naturally occurring neural computations during reward processing upon information about the connectivity of LH neurons. In addition, we used ex vivo patch-clamp experiments to explore the composition of GABAergic and glutamatergic LH inputs onto both DA and GABA neurons within the VTA. Building on our results from the recordings experiments, we utilized behavioral tasks to establish causal relationships between aspects of both reward seeking and feeding and the activation of distinct subsets of LH-VTA projections. Together, these data help us establish a model for how the components within the LH-VTA loop work together to process reward and how manipulating individual components can have profound effects on behavior.

rezultatai

Photoidentification of Distinct Components in the LH-VTA Circuit

In order to identify LH neurons that provide monosynaptic input to the VTA in vivo and observe their activity during freely moving behaviors, we used a dual-virus strategy to selectively express channelrhodopsin-2 (ChR2) in LH neurons providing monosynaptic input to the VTA (1 paveikslaiA ir S1). We injected an adeno-associated viral vector (AAV5) carrying ChR2-eYFP in a Cre-recombinase-dependent double-inverted open reading frame (DIO) construct into the LH to infect local somata and injected a retrogradely traveling herpes simplex virus (HSV) carrying Cre-recombinase into the VTA. Subsequent recombination permitted opsin and fluorophore expression selectively in LH neurons providing monosynaptic input to the VTA. To confirm our approach, we performed ex vivo whole-cell patch-clamp recordings in horizontal brain slices containing the LH and recorded from neurons expressing ChR2-eYFP, as well as neighboring LH neurons that were ChR2-eYFP negative (figūra 1B). Light-evoked spike latencies, measured from light-pulse onset to the peak of the action potential, ranged from 3–8 ms (figūra 1C). We also found that none of the non-expressing (ChR2-negative) cells recorded showed excitatory responses to photostimulation (n = 14; figūra 1C), despite their proximity to ChR2-expressing cells.

In order to perform optogenetically mediated photoidentification in vivo, an optrode was implanted into the LH to record neuronal activity during a sucrose-seeking task. In the same recording session, we provided several patterns of photostimulation to identify ChR2-expressing LH-VTA neurons (1 paveikslaiD ir S1). We examined the distribution of excitatory photoresponse latencies across all LH neurons displaying a time-locked change in firing rate in response to illumination and observed a bimodal distribution (figūra 1E). We observed a population of neurons during in vivo recordings with latencies in a range of 3–8 ms. This was identical to the latency range found in ChR2-expressing LH-VTA neurons when we recorded ex vivo. We termed these units “Type 1” units (1 paveikslaiC, 1E, and 1F). In addition, there was a distinct population of cells with ∼100 ms photoresponse latencies (1 paveikslaiE and 1G), and we termed these “Type 2” units. We also observed neurons that were inhibited in response to photostimulation of LH-VTA neurons (S2 paveikslas), and we termed these “Type 3” units. We compared the action potential duration (as measured from peak to trough) and mean firing rates of Type 1 and Type 2 units as well as those that did not show a photoresponse (figūra 1H). The distribution of action potential durations of Type 1 (figūra 1I) and Type 2 (figūra 1J) units shows that the majority of Type 1 units have an action potential duration less than 500 μs (84%; n = 16/19, binomial distribution, p = 0.002).

Although Type 1 units fit standard criteria to be classified as ChR2 expressing (Cohen et al., 2012, Zhang et al., 2013), it was unclear whether the longer latency photoresponse of Type 2 units was indicative of ChR2-expressing neurons that responded more slowly to photostimulation, or whether this effect was due to network activity. Given that the ChR2-expressing (Type 1) LH neurons project directly to the VTA, one possibility was that Type 2 neurons were receiving feedback from the VTA (figūra 1K). Another possibility was that Type 2 neurons were activated by axon collaterals from Type 1 neurons (figūra 1L). To differentiate between these two possible circuit models, we inhibited the VTA in conjunction with photoidentification in the LH.

Long Latency Photoresponses in LH Neurons Are Mediated by Feedback from the VTA

Based on our circuit models, we would expect distal inhibition to have no effect on the photoresponses of ChR2-expressing LH neurons. However, if photoresponsive, but non-expressing, LH neurons relied on feedback from the VTA to elicit a time-locked response to illumination (figūra 1K), we would expect an attenuation of photoresponses in these neurons upon VTA inhibition. We expressed ChR2 in LH-VTA cells as above, but this time also expressed enhanced halorhodopsin 3.0 (NpHR) in the VTA and implanted an optic fiber in the VTA in addition to the optrode in LH (figūra 2A). We delivered the same blue-light illumination patterns in the LH for all three epochs but also photoinhibited the VTA with yellow light in the second epoch (figūra 2A).

The photoresponses of Type 1 units to blue-light illumination in the LH were unaffected by photoinhibition of the VTA, which is consistent with ChR2 expression in Type 1 LH-VTA neurons (figūra 2B). In contrast, the majority of Type 2 units (87%; n = 13/15, binomial distribution, p = 0.004) showed a significant attenuation of photoresponses to blue-light pulses delivered in the LH upon photoinhibition of VTA neurons. The responses of Type 1 and Type 2 units during VTA photoinhibition were significantly different (chi-square = 7.64, p = 0.0057; 2 paveikslaiB and 2C). These differences can also be seen in the max Z scores during individual epochs (figūra 2D) and with the yellow-ON epoch normalized to the yellow-OFF epoch (figūra 2E). These data suggest that Type 2 LH neurons receive input (either directly or indirectly) from the VTA (figūra 1K) rather than via local axon collaterals (figūra 1L).

Distinct Encoding Properties of LH Neurons Either upstream or downstream of the VTA

Having identified these two distinct types of LH neurons in the LH-VTA loop, we wanted to examine naturally occurring neural activity during a sucrose self-administration task (figūra 3A). Mice were trained to perform nosepoke responses for a cue predicting sucrose delivery at an adjacent port (as in Tye et al., 2008). To allow us to differentiate neural responses to the nosepoke and the cue, the cue and sucrose were delivered on a partial reinforcement schedule, wherein 50% of nosepokes were paired with a cue and sucrose delivery.

Type 1 units showed phasic responses to sucrose port entry, as seen in a representative Type 1 unit (figūra 3B), as well as the population data for all Type 1 units (figūra 3C). The phasic responses of Type 2 units, however, mainly reflected responses to the reward-predictive cue (3 paveikslaiD and 3E). The normalized firing patterns of all recorded neurons (n = 198, divided into Type 1, 2, 3, and non-responsive units) are displayed for each task component: nosepokes paired with the cue, nosepokes in the absence of the cue, and sucrose port entry (figūra 3F). All Type 1 units that showed task-relevant phasic changes in activity (74%; n = 14/19) were either phasically excited or inhibited by sucrose port entry, with a small number also showing phasic inhibition to the reward-predictive cue (3 paveikslaiB, 3C, and 3G). In contrast, Type 2 units were more heterogeneous, with task-responsive neurons encoding the cue selectively (35%), the sucrose port-entry selectively (26%), or both the cue and port entry (12%; 3 paveikslaiD, 3E, and 3H). To illustrate the strength of responses of Type 1 and Type 2 units to task-related events, we plotted each cell on a three-dimensional plot according to Z score (figūra 3I). To show the distribution of phasic changes in firing to multiple task-related events on a qualitative level, we plotted the number of cells of each photoresponse type that fell into a given category (figūra 3J).

Different Components of the LH-VTA Circuit Represent Distinct Aspects of Reward-Related Behavior

Given the well-defined role of the VTA in reward-prediction error (e.g., the phasic reduction of DA neuron firing in response to the unexpected omission of a reward and the phasic excitation in response to unexpected reward delivery) (Schultz et al., 1997), we investigated whether LH neurons would encode the unexpected omission of a sucrose reward. To do this, we recorded the neural activity of photoresponsive neurons during the same cue-reward task in well-trained animals but randomly omitted 30% of sucrose deliveries following the cue (figūra 4A).

The majority of Type 1 units (88%; n = 15/17, binomial distribution, p = 0.001) were insensitive to reward omission (4 paveikslaiB and 4D), whereas a large subset of Type 2 units (67%; n = 12/18) showed a significantly different response to reward-presented and reward-omitted trials (4 paveikslaiC and 4D). We concluded that LH-VTA (Type 1) neurons encoded the action of entering the port, as these port-entry responses were persistent even upon reward omission (figūra 4D), in contrast to Type 2 units (chi-square = 10.9804, p = 0.0009).

To determine whether Type 1 responses to port entry were truly encoding the conditioned response (CR), as opposed to general reward-seeking or exploratory behavior, we recorded in untrained mice that had not yet acquired the task. In task-naive mice, we delivered sucrose to the port in the absence of a predictive cue (unpredicted reward delivery) and found that Type 1 units did not show phasic responses to port entry (4 paveikslaiE, 4F, and 4I), consistent with the model that Type 1 neurons encode the CR (figūra 4J).

Next, to determine whether Type 2 unit activity is consistent with a reward-prediction error-like response profile, we also recorded these neurons in well-trained animals during unpredicted reward delivery (figūra 4G). We found that a subset of Type 2 units responded to unpredicted sucrose deliveries (50%; 4 paveikslaiG–4I). Taken together, subsets of Type 2 units are sensitive to unexpected reward omission (4 paveikslaiC and 4D) and unpredicted reward delivery (4 paveikslaiG–4I), consistent with a reward-prediction error-like response profile.

Photostimulation of the LH-VTA Pathway Promotes Sucrose Seeking in the Face of a Negative Consequence

As we have shown above, Type 1 units represent a neural correlate of CR. Importantly, the increase in firing rate begins prior to CR, ramping up until the CR has been completed (3 paveikslaiB, 3C, and 4B). To determine whether activation of the LH-VTA pathway could promote CR, we wanted to test the ability of LH-VTA activation in driving CR in the face of a negative consequence. In wild-type mice, we expressed ChR2-eYFP or eYFP alone in LH cell bodies and implanted an optic fiber over the VTA (5 paveikslaiA ir S4). Conversely, to test the role of the LH-VTA pathway in mediating CR or feeding-related behaviors, we bilaterally expressed NpHR-eYFP or eYFP alone in LH cells and implanted an optic fiber above the VTA (5 paveikslaiA ir S4).

We designed a Pavlovian conditioning task in which food-deprived mice had to cross a shock grid to retrieve a sucrose reward (figūra 5B). In the first “baseline” epoch (with the shock grid off), we verified that each mouse had acquired the Pavlovian conditioned approach task. In the second (“Shock”) epoch, the shock grid delivered mild foot shocks every second. Finally, in the third epoch (“Shock+Light”), we continued to deliver foot shocks but also illuminated LH terminals in the VTA with blue light (10 Hz) in mice expressing ChR2 and matched eYFP controls and yellow light (constant) for mice expressing NpHR and their eYFP controls (figūra 5B).

We observed a significantly higher number of port entries per cue during the Shock+Light epoch and a significantly higher difference score (Shock+Light epoch − Shock-only epoch) in ChR2 mice relative to eYFP mice (figūra 5C ir Filmas S1). In contrast, photoinhibition of the LH-VTA pathway resulted in a significant reduction in port entries per cue and difference scores in the NpHR mice relative to eYFP mice (figūra 5D ir Filmas S2). Within-session extinction experiments during which cue presentations were not followed by sucrose deliveries showed similar trends in effect (S4 paveikslas).

Importantly, we wanted to determine whether the changes in sucrose seeking we had obtained were caused by changes in feeding-related behavior or sensitivity to pain. We observed that photoactivation of the LH-VTA projection significantly increased the time spent feeding in well-fed mice in the ChR2 group (figūra 5E). However, photoinhibition of the LH-VTA pathway did not significantly reduce feeding (figūra 5F), even though these animals were food deprived to enhance our ability to detect a reduction relative to the baseline epoch (compare to sated animals in figūra 5E). In neither the ChR2 (figūra 5G) nor NpHR group (figūra 5H) did we observe a difference in latency to tail withdrawal from hot water (Ben-Bassat et al., 1959, Grotto and Sulman, 1967), indicating that manipulating the LH-VTA projection was not altering analgesia.

LH Provides Both Glutamatergic and GABAergic Input onto VTA DA and GABA Neurons

To study the composition of the fast transmission components of LH inputs to the VTA that were eliciting these effects, we performed whole-cell patch-clamp recordings from VTA neurons in an acute slice preparation while optically activating LH inputs expressing ChR2-eYFP (6 paveikslaiA ir S5). Given that there is well-established heterogeneity within the VTA, including ∼65% DA neurons, ∼30% GABA neurons, and ∼5% glutamate neurons (Margolis et al., 2006, Nair-Roberts et al., 2008, Yamaguchi et al., 2007), we filled cells with biocytin while recording to allow for identification of cell type using post-hoc immunohistochemistry for tyrosine hydroxylase (TH; figūra 6B), in addition to recording the hyperpolarization-activated cation current (Ih) and mapping cell location (6 paveikslaiB ir S5).

First, we recorded in current-clamp during photostimulation of ChR2-expressing LH inputs and observed that 23 of 27 neurons showed a time-locked response to photostimulation of LH inputs (figūra 6C). The majority of DA neurons sampled in the VTA received a net excitatory input from the LH (56%), whereas another subset showed net inhibition (30%; figūra 6C). The spatial distribution of these DA neurons is mapped onto an atlas for horizontal slices containing the VTA (figūra 6D).

To establish the monosynaptic contribution of LH inputs to VTA DA neurons, we used ChR2-assisted circuit mapping, where voltage-clamp recordings were performed in the presence of tetrodotoxin (TTX) and 4-aminopyridine (4AP; Petreanu et al., 2007). Consistent with our observations from current-clamp recordings, we observed that the majority of recorded VTA DA neurons exclusively received excitatory monosynaptic input from the LH (67%), compared to VTA DA neurons that exclusively received inhibitory monosynaptic input (11%), or both (22%; 6 paveikslaiE ir S6).

We identified VTA GABA neurons by injecting a Cre-dependent fluorophore (AAV5-DIO-mCherry) into the VTA of VGAT::Cre mice and utilized mCherry expression to direct the recording of VTA GABA neurons (n = 24; figūra 6F). Forty-six percent of VTA GABA neurons responded with net excitation, whereas 54% responded with net inhibition, to photostimulation of ChR2-expressing LH inputs (figūra 6G). The spatial distribution of these cells is shown in figūra 6H. Upon examination of the monosynaptic input from the LH (as described above), we found that 18% of sampled GABA neurons received exclusively excitatory input and 9% received exclusively inhibitory input (figūra 6I). However, relative to VTA DA neurons, we found that more VTA GABA neurons received both excitatory AMPAR-mediated and inhibitory GABAAR-mediated monosynaptic input from the LH (73%; chi-square = 5.0505, p = 0.0246; 6 paveikslaiaš ir S6).

Distinct Roles of Glutamatergic and GABAergic Components of the LH-VTA Pathway in Behavior

Given that our ex vivo recordings provided evidence supporting robust input from both GABAergic and glutamatergic LH projections to the VTA, we next probed the role of each component independently. To do this, we used transgenic mouse lines expressing Cre-recombinase in neurons that expressed either vesicular glutamate transporter 2 (VGLUT2) or vesicular GABA transporter (VGAT). We injected AAV5-DIO-ChR2-eYFP or AAV5-DIO-eYFP into the LH of VGLUT2::Cre and VGAT::Cre mice and implanted an optic fiber over the VTA (S7 paveikslas). These animals were then run on each of the behavioral assays shown in figūra 5.

 

We did not observe any detectable differences in the number of port entries made per cue between mice expressing ChR2 or eYFP in the LHglostyti-VTA projection (figūra 7A) or in the LHGASR-VTA projection (figūra 7B). However upon video analysis, we noticed aberrant gnawing behaviors in the LHGASR-VTA:ChR2 group upon blue-light illumination (see Filmai S3 ir S4). In LHglostyti-VTA mice, although there was a trend toward a reduction in feeding upon photostimulation in the ChR2 group compared to the eYFP group, this was not statistically significant (figūra 7C). In contrast, we observed a robust increase in the time spent feeding in sated mice upon illumination in the LHGASR-VTA:ChR2 group relative to controls (figūra 7D ir Filmas S3). In neither group of animals was there an effect of light stimulation in the tail-withdrawal assay (7 paveikslaiE and 7F).

During the feeding task, as we did during the sucrose-seeking task, we again noticed aberrant feeding-related motor sequences that were not directed at food. We filmed a representative mouse in the LHGASR-VTA:ChR2 group in an empty transparent chamber, and upon 20 Hz photostimulation, we observed unusual appetitive motor sequences such as licking and gnawing the floor or empty space (Filmas S4). We quantified these “gnawing” behaviors during the feeding task in the wild-type LH-VTA (figūra 7G), LHglostyti-VTA (figūra 7H), and LHGASR-VTA (figūra 7I) groups and showed that LHGASR-VTA:ChR2 mice gnawed more than wild-type or LHglostyti-VTA:ChR2 mice when photostimulated, as compared to their respective eYFP groups (figūra 7J). We considered whether the aberrant feeding-related behaviors might be separated from appropriately directed feeding at lower frequencies. However, when we tested the LHGASR-VTA:ChR2 group with 5 Hz and 10 Hz trains of blue light, we observed a proportional relationship between stimulation frequency and both feeding and gnawing (figūra 7K).

Diskusija

Functional Components of the LH-VTA Loop

The LH projection to the VTA has been explored with electrical stimulation collision studies (Bielajew and Shizgal, 1986) and has long been hypothesized to play a role in reward processing (Hoebel and Teitelbaum, 1962, Margules and Olds, 1962), yet pinpointing this role has been a challenge. Here, we are providing a detailed dissection of how individual components of the LH-VTA loop process different aspects of a reward-related task.

Through the use of optogenetic-mediated phototagging (figūra 1), we have identified two separate populations of LH neurons: cells that send projections to the VTA (Type 1) and cells that receive feedback from the VTA (Type 2; figūra 2)—though these populations need not be mutually exclusive, as it is possible that LH neurons could both send and receive inputs to and from the VTA. Interestingly, we found that relatively few photoresponsive neurons fell outside the bimodal distribution encapsulating these two populations (S2 skaičiaiB ir 1E). Given this, in combination with the long latency delay in Type 2 photoresponses (∼100 ms), we speculate that there may be one dominant pathway contributing to the activity of Type 2 neurons. Additionally, because DA binds G protein-coupled receptors, the kinetics are slower than most glutamatergic synapses (Girault and Greengard, 2004) and may explain this cluster of 100 ms latency photoresponsive units. It is also possible that the VTA may provide indirect feedback through other distal regions, via excitatory intermediate regions such as the amygdala, or with disinhibition via the nucleus accumbens (NAc) or bed nucleus of the stria terminalis (BNST).

Interestingly, whereas photostimulation of Type 1 units evokes excitatory responses in Type 2 units, Type 1 and 2 units show distinct behavioral encoding properties. For example, the numbers of Type 1 and Type 2 units that selectively encode the reward-predictive cue are significantly different (n = 0/19 Type 1 versus n = 12/34 Type 2, chi-square = 8.67, p = 0.003). This paradoxical response pattern could be due to computational processes at an intermediate circuit element, such as the VTA, that may be playing an active role during the behavioral task but inactive during phototagging. Additionally, the behavioral state of the animal could influence how these data are processed.

 

 

Decoding Circuit Components in Reward Processing

Our reward omission experiments allowed us to distinguish between LH neural encoding of the CR and the consumption of the unconditioned stimulus (US). In these experiments, a subset of Type 2 units responded to the reward-predictive cue (CS) and the US and also showed a decrease in firing rate when expected rewards were omitted. Furthermore, a subset of Type 2 units also show phasic excitation upon unexpected reward delivery (4 paveikslaiG and 4H). These data are reminiscent of the way DA neurons in the VTA encode reward-prediction error (Cohen et al., 2012, Schultz et al., 1997). We speculate that VTA neurons may transmit reward-prediction error signals to a subset of LH neurons, which are well-positioned to integrate these signals for the determination of an appropriate behavioral output. Specifically, the LH is robustly interconnected with a multitude of other brain areas (Berthoud and Münzberg, 2011) and has been causally linked to homeostatic states such as sleep/arousal and hunger/satiety (Carter et al., 2009, Jennings et al., 2013).

A Causal Role for the LH-VTA Pathway in Compulsive Sucrose Seeking?

Compulsive reward-seeking behavior has primarily been discussed in the context of drug addiction, wherein a classic paradigm for compulsive drug seeking has been to examine the degree to which drug-seeking behavior persists in the face of a negative consequence, such as a foot shock (Belin et al., 2008, Pelloux et al., 2007, Vanderschuren and Everitt, 2004). We adapted this task for sucrose seeking to allow us to investigate whether activation of the LH-VTA pathway was sufficient to promote compulsive sucrose seeking. Given that a distinct difference between drug and natural reward is that drug rewards are not necessary for survival, there is controversy as to what behaviors would constitute compulsive sucrose- or food-seeking behavior. An alternative interpretation of our data is that activation of the LH-VTA pathway simply increases motivational drive or the urge to seek appetitive reinforcers. As the rates of obesity have increased in recent decades (Mietus-Snyder and Lustig, 2008), compulsive overeating and sugar addiction are prevalent conditions that are a major threat to human health (Avena, 2007). The feeding behavior in sated (fully fed) mice after activation of the LH-VTA pathway is reminiscent of eating behaviors seen in humans diagnosed with compulsive overeating disorder (or binge-eating disorder) (DSM-V).

It has been proposed that repeated actions lead to the formation of habits, which themselves lead to the compulsive reward seeking that characterizes addiction (Everitt and Robbins, 2005). Our finding that LH-VTA neurons only encode port entry after conditioning suggests that this pathway is selectively encoding a conditioned response, not just a motivated action. This is consistent with our observations that optically activating this projection can promote compulsive reward seeking in the face of a negative consequence (figūra 5C), as well as in the absence of need (as seen in sated mice, figūra 5E). This interpretation is further substantiated by our finding that photoinhibition of the LH-VTA pathway selectively reduces compulsive sucrose seeking (figūra 5D) but does not reduce feeding in food-restricted mice (figūra 5F). One of the greatest challenges in treating compulsive overeating or binge-eating disorders is the risk of impairing feeding behaviors in general. From a translational perspective, we may have identified a specific neural circuit as a potential target for the development of therapeutic interventions for compulsive overeating or sugar addiction without sacrificing natural feeding behaviors.

Composition of LH Input to the VTA

We show that in addition to a glutamatergic LH-VTA component (Kempadoo et al., 2013), there is also a significant GABAergic component in the projection (Leinninger et al., 2009), and that LH neurons synapse directly onto both DA and GABA neurons in the VTA (figūra 6). However, there is a difference in the balance of the excitatory/inhibitory input onto VTA DA and GABA neurons.

While we used immunohistochemical processing to verify the identity of VTA neurons, we also measured Ih, a hyperpolarization-activated inwardly rectifying non-specific cation current (Lacey et al., 1989, Ungless and Grace, 2012). The presence of this current has been widely used in electrophysiological studies to identify DA neurons, but it has been shown to be present only in subpopulations of DA neurons, delineated by projection target (Lammel et al., 2011). Although it has previously been proposed in a review by Fields and colleagues that “LH neurons synapse onto VTA projections to the PFC, but not those projecting to the NAc” (Fields et al., 2007), our data suggest that this controversy be reopened for further investigation. Even though we did observe a subset of DA neurons that received net excitation from the LH and possessed a very small Ih (consistent with mPFC- or NAc medial shell-projecting DA neurons), we also observed a subset of DA neurons that received net excitatory input and showed a large Ih (consistent with characteristics of DA neurons projecting to the lateral shell of the NAc; S5 paveikslas; Lammel et al., 2011). Conversely, VTA DA neurons that received a net inhibitory input showed a very small Ih or lacked this current, which is consistent with the notion that the LH sends predominantly inhibitory input onto VTA DA neurons projecting to the mPFC or the medial shell of the NAc. We also show that LH inputs can be observed in both medial and lateral VTA, suggesting that the LH provides inputs onto VTA neurons with diverse projection targets, as it is known that VTA projection target corresponds somewhat to spatial location along a medial-lateral axis (Lammel et al., 2008).

 

 

Excitation/Inhibition Balance in the LH-VTA Pathway

The role of the LH-VTA pathway in promoting reward has previously been ascribed to glutamatergic transmission in the VTA (Kempadoo et al., 2013), as the CaMKIIα promoter is often thought to be selective for excitatory projection neurons. However, our data clearly show that expressing ChR2 under the control of the CaMKIIα promoter also targets GABAergic projection neurons in the LH (figūra 6).

The behavior elicited by photostimulation of the LHGASR-VTA pathway was frenzied, mis-directed, and maladaptive (Filmas S4). One interpretation is that activation of the LHGASR-VTA pathway sends a signal to the mouse that causes the recognition of an appetitive reinforcer. An alternative interpretation is that the LHGASR-VTA pathway might drive incentive salience or an intense “wanting,” consistent with a signal underlying conditioned approach, but at a non-physiological level that produces this aberrant feeding-related behavior (Berridge and Robinson, 2003). Consistent with this, it is possible that activation of the LHGASR-VTA projection actually produces intense sensations of craving, or urges to feed. However, our experiments show that activation of LHGASR-VTA does not produce an increase in compulsive sucrose seeking, but this is likely due to the excessive gnawing and aberrant appetitive behaviors focused on non-food objects in the testing chamber. Although it is difficult to determine the experience of the mouse during this manipulation, it is clear that appropriately directed feeding-related behaviors require the coordinated activation of both the GABAergic and glutamatergic components of the LH-VTA pathway.

Išvada

Optogenetic and pharmacogenetic manipulations are powerful tools for establishing causal relationships, yet they do not reveal the endogenous, physiological properties of neural circuit elements. Our study unifies information about the synaptic connectivity, the naturally occurring endogenous function, and the causal role of the LH-VTA pathway, providing a new level of insight toward how information is integrated in this circuit. These results highlight the importance of examining the functional role of neurons by connectivity, in addition to genetic markers. LH-VTA neurons selectively encoded the action of reward seeking but did not encode environmental stimuli, whereas rewarding stimuli and reward-predictive cues were encoded by a discrete population of LH neurons downstream of the VTA. Furthermore, we have identified a specific projection that is causally linked to compulsive sucrose-seeking and feeding behavior. The heterogeneity in the LH-VTA projection is necessary for providing an adaptive balance between driving motivation and regulating appropriately directed appetitive behaviors. These findings provide insights relevant to pathological conditions such as compulsive overeating disorder, sugar addiction, and obesity

 
+

Extended Discussion

Autoriaus įnašai

E.H.N. and G.A.M. performed electrophysiological recordings and analyses for in vivo and ex vivo experiments, respectively. S.A.A., E.H.N., K.N.P., and C.A.L. performed behavioral experiments. R.W., K.N.P., C.A.L., and E.H.N. performed histological verification. R.N. provided HSV virus. K.M.T. and C.P.W. supervised experiments and trained experimentalists. E.H.N., G.A.M., S.A.A., and K.M.T. designed experiments. E.H.N. and K.M.T. wrote the manuscript; all authors contributed to the editing and revision of manuscript.

 

 

 

Padėka

We thank N. Golan, R. Thomas, M. Anahtar, G. Glober, and A. Beyeler for their assistance with immunohistochemistry. We would also like to thank C. Seo, and S. Kim for their contributions throughout the project and M. Wilson and P. Shizgal for helpful discussion. K.M.T. is a New York Stem Cell Foundation – Robertson Investigator and acknowledges funding from the JPB Foundation, PIIF, PNDRF, Whitehall Foundation, Klingenstein Foundation, NARSAD Young Investigator Award, Alfred P. Sloan Foundation, Whitehead Career Development Chair, NIH R01-MH102441-01 (NIMH), and NIH Director’s New Investigator Award DP2-DK-102256-01 (NIDDK). E.H.N. was supported by the NSF Graduate Research Fellowship, the Integrative Neuronal Systems Fellowship, and the Training Program in the Neurobiology of Learning and Memory. G.A.M. was supported by the Simons Center for the Social Brain Postdoctoral Fellowship. S.A.A. was supported by the Jeffrey and Nancy Halis Fellowship as well as the Henry E. Singleton Fund. C.A.L. was supported by the Integrative Neuronal Systems Fellowship and the James R. Killian Fellowship. R.W. was supported by the Netherlands Organisation for Scientific Research (NWO) RUBICON fellowship program.

 

 

Papildoma informacija

Movie S1. Related to Figure 5 

Wild-type animal expressing ChR2 in the LH with an optic fiber implanted over the VTA. The animal retrieves the sucrose consistently during the baseline epoch, but this is disrupted when the shock is introduced during the second epoch. However, photostimulation of LH-VTA projections in the third epoch causes the animal to retrieve the sucrose again. See Figure 5.
Movie S2. Related to Figure 5 

Wild-type animal expressing NpHR bilaterally in the LH with an optic fiber implanted over the VTA. The animal performs similarly in the first two epochs (see Movie S1) but is further deterred from retrieving sucrose in the third epoch with photoinhibition of the LH-VTA projection. See Figure 5.
Movie S3. Related to Figure 7 

In VGAT::IRES-Cre animals expressing ChR2 in GABAergic LH neurons with an optical fiber implanted over the VTA, stimulation of terminals in VTA produces voracious feeding. See Figure 7.
Movie S4. Related to Figure 7 

In VGAT::IRES-Cre animals expressing ChR2 in GABAergic LH-VTA projections, feeding evoked from LHGABA-VTA stimulation is often accompanied by “gnawing” behavior, which included aberrant appetitive motor sequences such as licking and gnawing the floor. We also observed in multiple mice from the LHGABA-VTA:ChR2 group the performance of a motor sequence as would be commonly observed when a mouse picks up a food pellet, holds it with both paws, and performs biting and chewing motions—except these actions were performed in the absence of any object. See Figure 7.

 

 

 

Figure thumbnail fx1

H