Kusiyanitsa kwa dopamine D2 ndi D4 receptor ndi tyrosine hydroxylase mRNA mu mbewa zoyenera, kapena zosagonjetsedwa, kuti asamadye kwambiri mafuta omwe amachititsa kunenepa kwambiri (2005)

Kafukufuku wapano adasanthula ubongo dopamine D2 ndi D4 receptor ndi tyrosine hydroxylase (TH) mRNA expression mu mafuta onenepa kwambiri omwe amachititsa kudya kwambiri (cDIO) ndi mbewa zosagwira (cDR) mbewa. Mbewa makumi awiri mphambu zisanu ndi zitatu zidapatsidwa chakudya chamafuta ambiri (HF: 40% ya mafuta kuchokera kwamafuta) kwamasabata 6 kenako amatchedwa cDIO (n = 8) kapena cDR (n = 8) mbewa malinga ndi kulemera kwambiri komanso kotsika kwambiri kwa thupi opeza, motsatana. Mbewa zisanu ndi ziwiri zinadyetsedwa zakudya zopanda mafuta (LF: 10% ya mafuta kuchokera kwamafuta) ndipo amagwiritsidwa ntchito ngati zowongolera. Pambuyo pakudya masabata makumi awiri, mafuta owoneka pa gramu yoyambira thupi anali okwera kwambiri mgulu la cDIO (chiŵerengero: 20, 0.25, ndi 0.09; P <0.04 cDIO vs. cDR ndi LF, motsatana). Pogwiritsa ntchito njira zochulukitsira mu situ hybridization, kuchuluka kwa D0.01 ndi D2 receptor ndi tyrosine hydroxylase (TH) mRNAs adayesedwa m'magawo angapo aubongo. Mbewa za cDIO zinali ndi chiwonetsero chachikulu kwambiri cha mawu a D4 receptor mRNA pachimake pa ma nucleus accumbens (AcbC, + 2%) ndi mbali zina za caudate putamen (CPu, 16% ndi 21%) poyerekeza ndi mbewa za CDR ndi LF. Mulingo wamanenedwe a D24 receptor mRNA mu AcbC ndi gawo lotulutsa mpweya wa CPu anali ogwirizana kwambiri ndi thupi lomaliza. Kafukufukuyu ndi woyamba kuwunika mwatsatanetsatane mawu a D2 mRNA mu ubongo wama mbewa pogwiritsa ntchito njira yosakanikirana. D4 receptor mRNA expression mu ventromedial hypothalamic nucleus (VMH) ndi gawo loyenda lamkati mwa septal nyukiliya inalinso yayikulu kwambiri mu mbewa za cDIO poyerekeza ndi mbewa za cDR ndi LF (+ 4% ndi + 31%; P <60). Mawu a mRNA anali okwera kwambiri m'chigawo chazigawo (+ 0.05%, P

PMID: 15857678

DOI: 10.1016 / j.molbrainres.2004.12.013