Mfundo
- • Kugwiritsa ntchito ma neuroni a LH GABAergic kumapangitsa munthu kukhala wokhutira komanso wogwira ntchito
- • Kupereka kwa maselo a LH GABAergic kumachepetsa kulemera, kugwiritsiridwa ntchito, komanso chifukwa
- • LH GABAergic neuronal subsets ndi maselo osiyana ndi MCH ndi maselo a Orx
- • Mu vivo Ca2+ Zithunzi zikuwonetsa zovuta zokhudzana ndi zovuta komanso zosokoneza
Kukonzekera makhalidwe ovuta kumveka, monga kufuna ndi kudya, ndikofunikira kuti moyo ukhalepo. The lateral hypothalamus (LH) ndi dera lachidziwitso chofunikira kwambiri pa zovuta komanso zovuta kuzichita, koma ngati ma neuroni omwe ali mkati mwa LH akuthandizira pazinthu zogwirizanazi sizidziwika. Pano, tikuwonetsa kuti optogenetic yosangalatsa ya kagawo kakang'ono ka LH GABAergic (Vgat-expressing) ma neuron amalimbikitsa machitidwe okhutira komanso omaliza, pomwe kuchotsedwa kwa majini amitsempha iyi kumachepetsa izi. Kuphatikiza apo, kuchuluka kwa LH kumeneku ndikosiyana ndi ma cell okhala ndi ma neuropeptides okhudzana ndi kudyetsa, mahomoni okhudzana ndi melanin (MCH), ndi orexin (Orx). Pogwiritsa ntchito vivo calcium imaging muufulu wokhala mbewa kuti tilembetse zochitika kuchokera kumaselo mazana, tidazindikira ma LH GABAergic neurons omwe amasankha magawo okhutira kapena okhutiritsa, koma osowa onse awiri. Njira zoyendetsedwa mwamphamvu, koma zolumikizana kwambiri, zimasiyanitsidwa ndi ma cell.
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