Stimularea erotică a stimulilor sub amisulprid și reboxetină: un studiu fMRI controlat cu placebo la subiecți sănătoși (2014)

Int J Neuropsychopharmacol. 2014 Oct 31;18(2). pii: pyu004. doi: 10.1093/ijnp/pyu004.

Graf H1, Wiegers M2, Metzger CD2, Walter M.2, Grön G2, Abler B2.

Abstract

FUNDAL:

Impaired sexual function is increasingly recognized as a side effect of psychopharmacological treatment. However, underlying mechanisms of action of the different drugs on sexual processing are still to be explored. Using functional magnetic resonance imaging, we previously investigated effects of serotonergic (paroxetine) and dopaminergic (bupropion) antidepressants on sexual functioning (Abler et al., 2011). Here, we studied the impact of noradrenergic and antidopaminergic medication on neural correlates of visual sexual stimulation in a new sample of subjects.

METODE:

Nineteen healthy heterosexual males (mean age 24 years, SD 3.1) under subchronic intake (7 days) of the noradrenergic agent reboxetine (4 mg/d), the antidopaminergic agent amisulpride (200mg/d), and placebo were included and studied with functional magnetic resonance imaging within a randomized, double-blind, placebo-controlled, within-subjects design during an established erotic video-clip task. Subjective sexual functioning was assessed using the Massachusetts General Hospital-Sexual Functioning Questionnaire.

REZULTATE:

Relative to placebo, subjective sexual functioning was attenuated under reboxetine along with diminished neural activations within the caudate nucleus. Altered neural activations correlated with decreased sexual interest. Under amisulpride, neural activations and subjective sexual functioning remained unchanged.

CONCLUZII:

In line with previous interpretations of the role of the caudate nucleus in the context of primary reward processing, attenuated caudate activation may reflect detrimental effects on motivational aspects of erotic stimulus processing under noradrenergic agents.

CUVINTE CHEIE:

amisulpride; erotic stimulus processing; fMRI; impaired sexual function; reboxetine

Introducere

Impaired sexual functioning is increasingly recognized as a side effect of psychopharmacological treatments affecting quality of life and adherence with treatment regimens (; ; ; ) with a growing interest in the underlying mechanisms. Since sexual dysfunction is also a common symptom of psychiatric disorders, like major depression, in and of itself, purely treatment-related effects are hard to investigate in patients affected (), requiring the investigation of treatment effects in healthy controls ().

Intact sexual functioning is based on a complex interplay of cerebral and spinal centers as well as hormonal, peripheral, and autonomic functions. The influence of different neurotransmitters and pharmacological principles on these interactions is still largely unclear. We have already investigated the neural correlates of sexual stimulation in healthy subjects using functional magnetic resonance imaging (fMRI) under subchronic treatment (7 days) with the selective serotonin reuptake inhibitor (SSRI), paroxetine, and with bupropion, a selective dopamine and noradrenaline (NA) reuptake-inhibitor. We demonstrated that subjectively impaired sexual functioning under the SSRI was accompanied by diminished neural activations within limbic and reward-related brain areas (), potentially related to increased reciprocal interactions between orbitofrontal cortex and the ventral striatum (). As expected from clinical observations (; ), impaired sexual functioning was not evident under bupropion, which led to even increased neural activations within brain regions related to salient and emotional stimulus processing (ie, sublenticular extended amygdala). Whereas these opposite effects of SSRI and dopamine and NA reuptake-inhibitor treatments were shown for direct erotic stimulation, decreased activation within fronto-parietal and cingulo-opercular attention networks was evident for both bupropion and paroxetine during expectation periods preceding erotic picture stimuli (). Thus, serotonergic and noradrenergic/dopaminergic medications showed divergent and concordant effects depending on the aspects of erotic stimulus processing investigated. In line with previous hypotheses (), the dopaminergic component of bupropion was held responsible for the increased activation upon direct visual erotic stimulation (), and the noradrenergic component was interpreted to mediate the effects on the expectation of erotic stimulation (). Supplementing our previous investigation with a new sample of subjects, we intended to further disentangle the neural effects of monoaminergic drugs, now applying a selective noradrenergic and an antidopaminergic treatment.

The selective noradrenalin reuptake inhibitor (SNRI), reboxetine, is an antidepressant with high selectivity towards the noradrenalin transporter and relatively low affinity for the serotonin transporter, monoamine, histamine, and acetylcholine receptors (). Therefore, it presents a well-suited vehicle to investigate the impact of NA on neural correlates of sexual stimulation. Effects of reboxetine on some aspects of sexual functioning have already been reported in patients (), with less detrimental effects compared with SSRIs (), particularly regarding sexual satisfaction, the ability to become sexually excited (), and achieving orgasm (). However, in line with case reports (; ; ), the product’s professional information reports a commonly increased risk to develop sexual side effects in terms of erectile dysfunction and prolonged/delayed and painful ejaculation for up to 10% of treated cases, with an increased rate of such side effects under higher dosages in depressed patients ().

The atypical antipsychotic drug amisulpride has high and selective affinity to postsynaptic D2 și D3 receptorilor (; ; ). The most common hypothesis to explain sexual dysfunction under antipsychotics (see for review) is blockade of dopamine D2 receptorilor () in the tuberoinfundibular pathway, with secondary increases of prolactin levels (). Accordingly, we expected decreased activation of dopaminergic neural correlates of sexual stimulation in particular under this specific drug.

Investigating the neural correlates of sexual stimulus processing under noradrenergic (reboxetine) and antidopaminergic (amisulpride) medication in healthy subjects, we intended to complement results from our previous investigations regarding the neural impact of serotonergic and dopaminergic principles in sexual functioning () and to further characterize the monoaminergic mechanisms regarding sexual functioning and stimulus processing within a new sample of healthy males.

materiale si metode

Participanții

We investigated 20 healthy, heterosexual, male, right-handed subjects under subchronic medication with amisulpride (AMS), reboxetine (REB), and placebo (PLA) in a randomized counterbalanced order. Exclusion of 1 subject from further analysis due to cerebral pathology (gliotic lesions) lead to a final sample of 19 participants (mean age 24.0 years, SD 3.1; range 20–32). Participants were recruited by personal communication or written advertisements at the campus of Ulm University. Prior to the study, each participant received a full medical evaluation, including medical history, physical examination, and a Structured Clinical Interview for DSM-IV Axis I Psychiatric Disorders. Participants with any current or past psychiatric disorder observed in the psychiatric exploration with open questions by one of the study physicians (H.G. or B.A.) or with symptoms coded as “present” or “subthreshold” in any of DSM-IV Axis I Psychiatric Disorders modules would have been excluded from the study. Laboratory blood tests and electrocardiograms were performed to exclude renal, hepatic, or cardiac pathology. Further exclusion criteria were any serious general medical condition, current or past neurological illness, relevant baseline sexual dysfunction or sexual disorders, use of illegal drugs, and excessive consumption of caffeine or alcohol. Upon recruitment, baseline depressive symptoms were assessed by the German version () of the Center for Epidemiologic Studies Depression Scale (). The Massachusetts General Hospital Sexual Functioning Questionnaire (MGH-SFQ; ) was administered to evaluate baseline sexual interest, sexual arousal, ability to achieve orgasm, ability to achieve and maintain an erection, and overall sexual satisfaction prior to the study. According to the study protocol, the questionnaire was modified to assess changes in subjective sexual functioning only over the past week of medication (). Upon inclusion, subjects presented with sum scores of this specific questionnaire around 10 (mean 10.7; SD 1.7), indicating unimpaired subjective sexual functioning.

The study was approved by the local ethical committee of Ulm University, and all participants gave written informed consent conforming to the Declaration of Helsinki.

Study Design and Procedures

Within a randomized, double-blind, placebo-controlled, within-subject crossover design, subjects received 200mg AMS (100mg twice per day), 4mg REB (2mg twice per day), and PLA (twice per day) for 7 days each, separated by a wash-out phase of at least 2 weeks. As with our previous study protocol (), this new sample of subjects was investigated on 3 different occasions, and the fMRI scans took place on the seventh day of medication, 2 hours after intake of the last capsule. Subjects were asked to refrain from alcohol parallel to the study medication and especially for 3 days prior to fMRI. They were also asked to refrain from coffee on the day of the scans. To warrant drug exposure and adherence, blood samples were obtained after each scan (about 3 hours after drug intake) and analyzed after completion of the whole study. The average plasma amisulpride level was 137.7ng/mL (SD 54.8), and the mean plasma reboxetine level was 75.7ng/mL (SD 28.9). Blood levels within the expected range were detected in each of the 19 subjects for both drugs, indicating that adherence to drug intake was consistent across subjects.

Additional Questionnaires

Sexual functioning during the past week with drug or placebo intake was assessed using the MGH-SFQ after each scan. The MGH-SFQ consists of 5 questions with ratings from 1 to 6. Cumulative ratings range from 5 (minimal value: improvement of sexual functioning) over 10 (sexual functioning unchanged compared with normal) to 30 (maximal value: sexual functioning markedly impaired compared with normal). Ratings of <2 for single questions or a cumulative score >10 indicate subjectively impaired sexual functioning (for details, see ; ). Side effects of medication were assessed by one of the study physicians (H.G. or B.A.) within a medical interview with open questions and a structured part (UKU side-effects scale, ) on each session. Sedative effects of the medication were assessed with the Stanford Sleepiness Scale (SSS; ). Repeated-measures analysis of variance (ANOVA) and post-hoc Newman Keuls tests were computed to analyze questionnaire results.

fMRI Stimuli

Erotic and neutral video clips were used for prolonged visual stimulation within a standard block design as in our previous experiment (). Erotic video clips were extracted from commercial adult films and depicted sexual interaction (petting, oral sex, vaginal intercourse) between 1 man and 1 or 2 women. Neutral video clips showed men and women in emotionally neutral, nonerotic interactions. Erotic and neutral stimuli were matched for color, the number and gender of subjects interacting, length of interaction, and whether the depicted subjects were clothed or naked. Nine video clips of each of the 2 conditions were presented for 20 seconds each, separated by a 20-second fixation period, resulting in a total paradigm length of 720 seconds. Video clips were presented by magnetic resonance-compatible video goggles in a pseudo-randomized order with a maximum of 2 consecutive clips of the same condition. The order of the same video segments was counterbalanced across subjects and medications.

fMRI Acquisition

T1 anatomical volume images (1x1x1-mm voxels) and functional magnetic resonance images were acquired using a 3T head-only magnetic resonance imaging system (Siemens Magnetom Allegra, Erlangen, Germany). Twenty-three transversal slices were acquired with an image size of 64×64 pixels and a field of view of 192mm. Slice thickness was 3mm with a 0.75-mm gap, resulting in a voxel size of 3x3x3.75mm.

Slices were oriented steeper than the bicommissural line at an angle of −15° between transversal and coronal planes to minimize the risk of susceptibility in basal structures of the brain including subcortical regions of interest (amygdala, basal ganglia, and inferior prefrontal regions). Because of the reduced number of slices as a consequence of the rather short repetition time (TR) of 1.5 seconds, there was no full brain coverage. Functional images were recorded using T2*-sensitive gradient echo echo-planar imaging measuring changes in BOLD contrast. A total of 487 volumes was obtained during viewing of video clips at a TR of 1500 milliseconds (TE 35 milliseconds, flip angle 90°). The first 7 images were acquired to allow for T1 saturation effects and later discarded. Two additional image acquisitions at the very beginning of each sequence and not saved to disk added to the T1 equilibration time (3 seconds). At the end of scanning, a high resolution T1-weighted structural image was obtained by administering a magnetization prepared rapid acquisition gradient echo sequence (TR = 2300 milliseconds, TE = 3.93 milliseconds, inversion time = 1100 milliseconds, flip angle = 12°, FoV = 256mm, matrix size: 256 x 256, voxel volume = 1mm3, slice orientation: sagittal; scan time = 517 seconds).

Analiza fMRI

Image preprocessing and statistical analyses were carried out using Statistical Parametric Mapping (Wellcome Department, London, UK) with a random effects model for group analyses. Data from each session were preprocessed including slice-timing, realignment, and normalization into a standard template (Montreal Neurological Institute) with a spatial resolution of 2x2x2mm3. Smoothing was applied with an 8-mm FWHM isotropic Gaussian kernel. Intrinsic autocorrelations were accounted for by AR(1), and low frequency drifts were removed via high-pass filtering.

Similar cu , first-level analyses were performed for each subject. According to the general linear model, we defined 2 regressors to analyze each of the 2 types of video stimuli (erotic, nonerotic). Video blocks were modeled as timely extended events of 20 seconds and convolved with the hemodynamic response function. The 6 realignment parameters modeling residual motion were also included in the individual models. None of the time series contained movements of 1mm or more in any direction or rotations of >1 degree between consecutive volumes. The individual contrast images for erotic and nonerotic conditions were then included in a second-level group analysis using an ANOVA model with condition (erotic/nonerotic) as the first factor. Treatment (PLA/AMS/REB) was added as a second factor with 3 levels to test on significant interaction effects of treatment on the contrast of erotic vs nonerotic stimuli. A third factor modeled the subject-related variance.

Treatment effects were analyzed by computing 1-tailed condition-by-treatment interactions to account for 1 active compound and 1 active condition vs 1 placebo and 1 control condition (erotic, nonerotic; placebo, verum). Taking the one-sidedness of the t-contrasts into account, effects were considered significant at a statistical threshold of P<0.0025, uncorrected at the voxel level and a cluster extent of at least 419 contiguously significant voxels corresponding to a level of P<0.05, familywise error (FWE) corrected on the cluster level.

To investigate significant correlations between fMRI treatment effects and individual subjective sexual functioning, we further computed a multiple regression analysis incorporating each of the 5 MHG-SFQ subscales within the mask of the significant condition-by-treatment interaction. The dependent variables were the individual parameter estimates of condition-by-treatment interaction from contrasting placebo against reboxetine. Regressors were each individual’s difference score between placebo and reboxetine for each of the 5 subscales. One-tailed t-contrasts were used to test for significant regression effects. Effects were considered significant at a level of P<0.0025 at the voxel level and a cluster extent of at least 16 contiguously significant in-mask voxels, corresponding to a level of P<0.05, FWE corrected on the cluster level.

REZULTATE

chestionare

An average Center for Epidemiologic Studies Depression Scale sum-score of 8.0 (SD 6.04) indicated no relevant depressive symptoms in the participants.

Side effects as assessed by the UKU side effect scale did not appear for the following variables: increased duration of sleep, increased dream activity, dystonia, rigidity, hypokinesia/akinesia, hyperkinesia logic, tremor, akathisia, epileptic seizures, paraesthesias, increased salivation, diarrhea, micturition disturbances, polyuria/polydipsia, orthostatic dizziness, palpitations/tachycardia, increased tendency to sweating, rash, pruritus, photosensitivity, increased pigmentation, weight gain, weight loss, galactorrhoea, and gynaecomastia. At least one indication of a side effect under either drug was given for the following items, whereas between-treatment differences were not significant: concentration difficulties (F(2,36)=0.55; P=0.582), asthenia/lassitude/increased fatigability (F(2,36)=2.15; P=0.131), sleepiness/sedation (F(2,36)=1.61; P=0.214), failing memory (F(2,36)=1.00; P=0.378), depression (F(2,36)=1.31; P=0.283), emotional indifference (F(2,36)=1.18; P=0.320), accommodation disturbances (F(2,36)=0.19; P=0.827), nausea/vomiting (F(2,36)=0.24; P=0.788), constipation (F2,36)=0.08; P=0.924), and (tension) headache (F2,36)=0.12; P=0.888). Significant between-treatment differences were observed for the 3 side effects: reduced salivation (F(2,36)=4.58; P=0.017), inner unrest (F(2,36)=3.71; P=0.034), and reduced duration of sleep (F(2,36)=6.87; P=0.003). Post-hoc testing showed decreased salivation under reboxetine (REB) compared with placebo (P=0.033) and amisulpride (P=0.013), whereas amisulpride (AMS) did not differ from placebo (P>0.05). Inner unrest was also more evident under REB when compared with AMS (P=0.032) but not relative to placebo (P=0.067); again, AMS and placebo did not differ (P>0.05). Reduced duration of sleep was reported under REB compared with placebo (P=0.006) and AMS (P=0.004), whereas duration of sleep was not significantly different under AMS relative to placebo (P>0.05). It is of note in this context that an ANOVA for repeated measurements showed no significant treatment effect on sedation or sleepiness immediately after fMRI scanning (SSS; F(2,36)=1.47; P=0.244; for details see Table S1).

The mean overall score in the MHG-SFQ upon enrollment was 10.7 (SD 1.70) and 11.7 (SD 2.50) under placebo. Paired t-testing revealed no significant difference between placebo and enrollment scores (t(18)=−1.55; P=0.138), and MGH-SFQ-data upon enrollment were not considered further. Treatment effects on sum-scores in the MHG-SFQ were significant (F(2,36)=8.10; P=0.001). Post-hoc tests (Newman Keuls) confirmed more impaired sexual functioning under the SNRI reboxetine compared with both placebo (P=0.002) and amisulpride (P=0.003). Sexual functioning under the antidopaminergic drug amisulpride did not differ from placebo (P=0.850). Considering the different subscales (Tabelul 1), reboxetine had detrimental effects relative to either placebo or amisulpride for sexual arousal, orgasm, and the ability to achieve/maintain an erection. Reduced sexual satisfaction was also observed under reboxetine relative to amisulpride, but not when compared with placebo. Comparisons between amisulpride and placebo did not reveal significant differences in any of the subscales (Figura 1A-B).

Tabelul 1. 

Results from the ANOVA with the Factor “Treatment” (Reboxetine/Amisulpride/Placebo) on Group-Averaged Ratings for Each Subscale of the MGH-SFQ.
Figura 1. 

A, Mean total scores (SD) in the Massachusetts General Hospital Sexual Functioning Questionnaire (MGH-SFQ). Overall, subjective sexual functioning was significantly (P<0.05) impaired under reboxetine compared with placebo and amisulpride. Sexual ...

Rezultate fMRI

Efectele principale

Replicating previous results (), main effects of erotic visual stimulation under placebo (erotic minus nonerotic video clips) in the present sample showed significantly (P< 0.05 FWE cluster level corrected) increased neural activations in the superior and inferior temporal gyrus, middle occipital gyrus, inferior parietal lobule, inferior frontal gyrus, precentral gyrus, insula, hippocampus, midbrain (comprising parts of the substantia nigra), putamen, and caudate nucleus.

Treatment Effects

Significant condition-by-treatment interactions were found for a cluster comprising the right caudate nucleus and parts of the right anterior putamen and the lentiforme nucleus. Post-hoc tests confirmed that these effects were related to attenuated neural activations under reboxetine compared with placebo (see Tabelul 2; Figura 2A-B). The inverted t-contrast testing on increased neural activation under reboxetine against placebo did not reveal any significant effects. Furthermore, no significant results were obtained when contrasting placebo against amisulpride or amisulpride against reboxetine, even at a more lenient statistical threshold of P< 0.025 and without cluster corrections.

Tabelul 2. 

Significant Treatment-by-Condition Interaction When Contrasting Placebo Minus Reboxetine for the Contrast of Erotic Minus Non-erotic Stimulation (ANOVA; P < 0.0025, Uncorrected at the Voxel Level and a Cluster Extent of at Least 419 Contiguously ...
Figura 2. 

A, Significant placebo-by-reboxetine interaction within the right caudate nucleus depicted in sagittal and transversal slices at the peak voxel (P<0.0025, uncorrected at the voxel level and cluster extent of at least 419 contiguously significant ...

Analiza corelațiilor

A multiple regression analysis was computed to test for relations of subjectively experienced changes in sexual functioning and differential fMRI activations under the SNRI reboxetine against placebo. We revealed a significant partial correlation within the ventral part of the right caudate nucleus (mean partial correlation coefficient r= 0.709, P = 0.05 FWE corrected at cluster level; number of voxels: 22; peak Z-value: 3.10; x, y, z =10, 22, 4) with the MGH-SFQ subscale “sexual interest” indicating that decreased neural activation was accompanied by decreased sexual interest (Figura 3). None of the other MGH-SFQ subscales correlated significantly with fMRI activation differences.

Figura 3. 

Results of the multiple regression analysis of fMRI data and MGH-SFQ subscales as regressors within a mask comprising the voxels with a significant placebo-by-reboxetine interaction (actual cluster size: 565). A significant correlation (mean partial correlation ...

Discuție

Expanding our previous investigation () on neural correlates of sexual stimulation under serotonergic and dopaminergic medication, in the present study we focused on the effects of noradrenergic and antidopaminergic medication within a new sample of subjects. Within a double-blind, placebo-controlled, crossover design, we investigated 19 healthy male volunteers using an established erotic video clip task under administration of the noradrenergic drug reboxetine, the antidopaminergic agent amisulpride, and placebo. Reboxetine reduced subjective ratings of sexual functioning compared with both placebo and amisulpride, whereas ratings of sexual functioning did not change under amisulpride. Specifically, sexual arousal and the ability to achieve orgasm and achieve and maintain an erection were affected under reboxetine relative to placebo and amisulpride.

fMRI during erotic stimulation replicated significant activations of brain regions that have already been reported in previous studies (; ; ; ; ; ; ), thus supporting the reliability of the functional challenge. Significant treatment-by-condition interactions were exclusively related to reboxetine (SNRI) in terms of decreased activation of the right caudate nucleus compared with placebo and amisulpride. Moreover, activation changes in the ventral part of the caudate nucleus were correlated with changes in subjective ratings of sexual interest. Along with unchanged behavioral data, no significant treatment effects on neural activation upon erotic stimulation were observed for AMS relative to PLA.

Given the role of dopamine in processing sexual stimuli (; ) and the antidopaminergic properties (; ) of amisulpride, more pronounced effects were expected than observed (Figura 2B). This absence of greater effects may most likely associate with dose-dependent pharmacological properties of the drug (). Antipsychotic effects of amisulpride were reported for high dosages from about 400 to 600mg/d, resulting in reliable D2 receptor occupancy (). However, reports have suggested antidepressant efficacy at lower dosages of 50 to 200mg/d (as used in our study) by blocking presynaptic dopamine autoreceptors (; ; ). These mild prodopaminergic effects may account for the lack of impaired sexual functioning in our sample. As another possible explanation, present dosage of amisulpride may not have sufficiently increased plasma prolactin levels that are commonly linked to amisulpride-related sexual dysfunction () and may exert their effects via peripheral rather than central dopaminergic pathways.

Because the noradrenergic agent reboxetine has been reported favorably regarding sexual dysfunction associated with antidepressive medication (; ), we rather expected less detrimental effects of this drug than observed. However, previous reports of prolonged orgasm (), erectile dysfunction, and spontaneous ejaculation (; ) as well as anorgasmia () under reboxetine are in line with present results. Assuming an inverted u-curve relationship, an increase of noradrenergic neurotransmission beyond the optimum of an already balanced, healthy NA system may relate to the decrease in sexual functioning, similar to previous results where NA overstimulation negatively affected cognitive functioning in healthy subjects ().

Functionally, significant treatment effects on neural activations were limited to the right caudate nucleus with attenuated neural activations under the SNRI reboxetine compared with placebo or amisulpride. Activation of the caudate nucleus has been consistently involved in aspects of social behavior, romantic love, and erotic stimulus processing (; ; ; ) with a lateralization to the right as in our study (; ). Furthermore, in previous studies, measures of increasing penile turgidity and erection have been associated with elevated neural activations of the caudate nucleus (; ). During erotic stimulation, caudate nucleus activation has been linked to goal-directed behavior and reward (). Although activity within the most ventral parts of the striatum, particularly those comprising the nucleus accumbens, are commonly associated with the expectation and receipt of incentives, a recent investigation in healthy subjects () could associate the different aspects of reward vs motivational processing with differential activation along the caudate. Dorsal striatal activation increased with motivation while increasing activation of more ventral parts of the nucleus increased with reward. Therefore, our finding of decreased caudate activation under reboxetine and the correlation of decreased activation with diminished sexual interest may express predominantly motivational aspects of erotic stimulation. In line with this interpretation, a recent study on the effects of reboxetine at similar dosages and also in healthy subjects revealed unaffected ventral striatal activity upon processing of rewarding food stimuli ().

Since the striatum in humans is not significantly innervated by noradrenergic neurons (), attenuated neural activations within the caudate under the SNRI most likely reflect an indirect effect of increased NA levels. Reciprocal interactions between the dopamine and NA system () and the inhibitory action of NA on dopaminergic neurons () may offer an explanation here.

Limitări

In the present study, only male subjects were investigated to avoid biasing results due to hormonal variations related to the menstrual cycle in women (eg, ). Therefore, the present results cannot easily be transferred to a female sample, which would require an additional study explicitly controlling for hormonal state. The reduced duration of sleep under reboxetine as an expected side effect of a noradrenergic agent might constitute a possible factor to interfere with data acquisition under a functional challenge. However, results from the SSS immediately obtained after fMRI scanning did not indicate that sleepiness or sedation would have played a significant role. Compared with subjective assessments upon enrollment, we observed a slight although insignificant increase in MGH-SFQ sum scores already under placebo, which may indicate that information about putative side effects could have triggered some form of expectation effects even under placebo. Such expectation effects, however, would have opportunistically influenced differences between verum and placebo, only if these effects would have reduced MGH-SFQ scoring under placebo leading to a biased increase between verum and placebo. The slight increase in MGH-SFQ scoring speaks against this possibility.

Although questionnaire data may well inform about subjective experiences with sexual functioning, objective measurements of aspects of sexual response, eg, penile turgidity, should be considered additionally in future studies on the same topic. In a similar vein, an objective measurement of alcohol intake could be useful to complement verbal reports on adherence to the study protocol particularly in an investigation in young and healthy university students. Finally, further investigations might consider different dosages of the same drug in one experiment to investigate dose-dependent effects that might explain the absence of an interaction effect under the relatively low dose of amisulpride.

Concluzie

Within a double-blind, placebo-controlled, crossover design, we investigated the neural correlates of erotic stimulus processing in healthy subjects under the noradrenergic antidepressant reboxetine and the dopamine antagonist amisulpride to examine drug effects independent from otherwise illness-related alterations in sexual functioning under these medications. Low-dose amisulpride did not alter subjective ratings of sexual functioning, and accordingly, neural activations remained unaffected, which may relate to possible prodopaminergic effects under low dosages of this drug. For the noradrenergic agent reboxetine, we observed subjectively impaired sexual functioning in healthy subjects accompanied by attenuated neural activations within the caudate nucleus that may reflect diminished motivational aspects of erotic stimulus processing under noradrenergic agents.

Material suplimentar

For supplementary material accompanying this paper, visit http://www.ijnp.oxfordjournals.org/

Declarație de interes

All authors declare that there are no competing financial interests or funding in relation to the work described. C. Metzger and M. Walter were supported by SFB-779.

recunoasteri

We thank Prof. Dr C. Hiemke and his staff at the University of Mainz (Germany), Department of Psychiatry and Psychotherapy, for measuring drug serum levels.

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