Faʻamatalaga o suiga o le molécule i methamphetamine-activated Neurons mai le tasi rat i fafo e faʻaaogaina ai le faʻaogaina o le masini (FACS) (2013)

J Neurochem. 2014 Ian;128(1):173-85. doi: 10.1111/jnc.12381. Epub 2013 Aokuso 21.

Liu QR1, Rubio FJ, Bosser JM, Marchant NJ, Fanous S, Hou X, Shaham Y, Faamoemoe BT.

lē faʻatino

Methamphetamine ma isi vailaʻau e faʻagaoioia se vaega itiiti o neu uma i totonu o le faiʻai. Na matou faia muamua se auala e faʻavasega ai le faʻavasegaina o le cell fluorescence (FACS) e faʻaalia ai suiga mole mole faʻaosoina filifilia i neu faʻagaoioia e faʻaalia ai le neural activity marker Fos. Ae ui i lea, o lenei metotia e manaʻomia ai le tuʻufaʻatasia o faʻataʻitaʻiga mai le tele o iole. Ua matou faamatalaina nei se auala faʻavae faʻavae FACS e faʻaalia ai suiga mole mole i Fos-faʻaalia dorsal striatal neurons mai se isumu e tasi e faʻaaoga ai se taʻiala muamua-faʻateleina.

Fos ma NeuN (se neuronal marker) immunohistochemistry fa'ailoa mai e 5-6% o dorsal striatum neurons na fa'agaoioia 90 min ina ua mae'a tui methamphetamine (5 mg / kg, ip) ae itiiti ifo i le 0.5% o neu na fa'agaoioia e tui saline.

Na matou faʻaogaina le FACS e tuʻueseese ai neu faʻaigoaina NeuN i neu Fos-positive ma Fos-negative ma iloilo faʻamatalaga mRNA e faʻaaoga ai le RT-qPCR mai le laʻititi e lima Fos-positive neurons. Methamphetamine fa'aosoina 3-20-fa'ateleina fa'atuputeleina vave o kenera arc, homer-2, c-fos, fosB, ma ona isoforms (ΔfosB ma se tala fou o le isoform ΔfosB-2) i le Fos-positive ae le o Fos-negative neurons. O le amataga o le gene mRNA induction na 10-pa'i i lalo pe leai pe a su'esu'eina i fa'ata'ita'iga e le'i fa'avasegaina mai le tasi dorsal striatum homogenates. O a matou metotia faʻaleleia e mafai ai ona suʻesuʻeina suiga faʻapitoa mole mole i neu o loʻo faʻagaoioia e vailaʻau poʻo faʻamatalaga e fesoʻotaʻi ma vailaʻau i faʻataʻitaʻiga lavelave o vaisu.

Methamphetamine ma isi vailaʻau e faʻagaoioia se vaega itiiti o neu uma i totonu o le faiʻai. Matou te lipotia iinei se auala faʻaleleia e faʻaalia ai suiga mole mole faʻaosoina filifilia i neu faʻagaoioia e faʻaalia ai le faʻailoga o le neural activity Fos. Na matou faʻaogaina le FACS faʻatasi ai ma le faʻaogaina muamua o le PCR e suʻesuʻe ai faʻamatalaga faʻamaʻi o le methamphetamine mai le itiiti ifo i le 5 Fos-faʻaalia neu mai le tasi rat dorsal striatum. Methamphetamine fa'aosoina 3-20-fa'ateleina fa'atuputeleina vave vave (IEGs) i Fos-lelei ae le o Fos-negative neu. Fa'atatau i le PCR mua'i fa'atuputeleina e mafai ai ona su'esu'e suiga mole mole tulaga ese i neu ua fa'agaoioia e vaila'au po'o fa'ailoga e feso'ota'i ma vaila'au i fa'ata'ita'iga lavelave o vaisu.

Lolomiina 2013. O lenei tusiga o se galuega a le Malo o Amerika ma o loʻo i totonu o le lautele lautele i Amerika.