LIPOTSO: Teko ea pele e bontšang hore dopamine e ama maemo a DeltaFosb.
Eur J Neurosci. 1996 Feb; 8 (2): 365-81.
Doucet JP, Nakabeppu Y, Bedard PJ, Hope BT, Nestler EJ, Jasmin BJ, Chen JS, Iadarola MJ, St-Jean M, Wigle N, Blanchet P, Grondin R, Robertson GS.
mohloli o moholo
Lefapha la Pharmacology, Yunivesithi ea Ottawa, Ottawa, Ontario, Canada K1H 8M5.
inahaneloang
Ho sebelisa anti-antibody e amohelang lihlahisoa tsa litho tsohle tse tsebahalang tsa lelapa la fos la liphatsa tsa lefutso tsa kapele, ho ile ha bonts'oa hore tšenyo ea tsela ea nigrostriatal ka lisele tsa 6-hydroxydopamine (6-OHDA) tsa mokotla o ka pele oa boko li hlahisa nako e telele (> 3) months) ho phahama ha Fos-like immunoreactivity ho striatum. Re sebelisa mekhoa ea ho khutlisetsa pampiri morao, re bontšitse pejana hore keketseho ena ea ho itšireletsa mafung e kang Fos e fumaneha haholo ho li-neuron tsa striatal tse lebisang ho globus pallidus. Thutong ea hajoale, li-blots tsa Bophirima li entsoe ka likarolo tsa nyutlelie tse tsoang ho li-rats tse sa sisinyeheng tsa 6-OHDA ho fumana mofuta oa li-protein tsa Fos-immunoreactive tse ikarabellang bakeng sa keketseho ena. Hoo e ka bang libeke tse 6 kamora leqeba la 6-OHDA, polelo ea li-antigen tse peli tse amanang le Fos tse nang le limolek'hule tse hlakileng tsa 43 le 45 kDa li ile tsa matlafatsoa ho striatum e senngoeng. Tsamaiso e sa foleng ea haloperidol e boetse e fana ka maikutlo a phahameng a li-antigen tse amanang le Fos, a fana ka maikutlo a hore ho kenella ha bona kamora ho tlosoa ha dopaminergic ho sebetsoa ka ho fokotsa ts'ebetso ea li-receptor tsa D2-joalo ka dopamine.
Ho thibela pelehi ka bochabela ho sebelisa antibody e ananelang N-terminus ea FosB ho bonts'itse hore li-antigen tse amanang le 43 le 45 kDa Fos tse kentsoeng ke dopaminergic denervation le nako ea ts'ebetso ea haloperidol e sa ts'oaneng li ka amana le mofuta o fokotsoeng oa FosB o tsejoang e le deltaFosB. Tumellanong le tlhahiso ena, liteko tsa ho khutlisa tlhahlobo ea morao-rao li netefalitse hore ts'ireletso ea deltaFosB e tšoanang le ea li-striatum tse sa utloeng litsebeng e fumaneha haholo litsing tsa methapo ea kutlo.
Liteko tsa Gel shift li bonts'itse ts'ebetso e tlamang ea AP-1 e kopantseng striata e nang le protheine (F) ea FosB, e fana ka maikutlo a hore maemo a ntlafatsang a deltaFosB a ka kopanya litlamorao tse ling tsa litlamorao tsa dopamine tsa nako e telele ho mofuta oa AP-1 o laoloang ke li-neurons tsa striatopallidal. Ka lehlakoreng le leng, tsamaiso e sa fetoheng ea D1-like receptor agonist CY 208243 ho 6-OHDA-lesioned rats e ntlafalitse haholo ts'ebetso ea "delunFosB-like immunoreactivity" ea li-striatal neurons tse kenelletseng ho li-nigra tse kholo. Immunostun ea Western blot e senoletse hore deltaFosB mme, ho isa tekanyong e nyane, FosB e phahamisoa ke tsamaiso ea agonist e sa feleng ea D1. Ka bobeli tekanyetso ea reverse transcriptase-polymerase ketane le ts'ireletso ea ribonuclease e bontšitse hore maemo a deltafosB mRNA a ntlafalitsoe haholo ts'ebetsong e senotsoeng ke tsamaiso ea agonist e sa foleng ea D1.
Qetellong, re hlahlobile litlamorao tsa taolo e sa foleng ea li-ag1 tse kang D2 le D1-like dopamine receptor agonists ho striatal deltaFosB expression ho 4-methyl-1,2,3,6-phenyl-1-tetrahydropyridine (MPTP) primate ea lefu la Parkinson . Ho litšoene tse fetoletsoeng Parkinsonian ke MPTP, ho bile le keketseho e nyane ea li-protein kapa li-protein tsa deltaFosB, ha nts'etsopele ea dyskinesia e hlahisoang ke tsamaiso e sa foleng ea D2-like agonist e ne e tsamaea le keketseho e kholo ea li-protein kapa li-protein tsa DeltaFosB. Ka lehlakoreng le leng, tsamaiso ea DXNUMX-like agonist cabergoline e sebelisang nako e telele, e fokotsang matšoao a Parkinsonia ntle le ho hlahisa dyskinesia e fokolitse maemo a deltaFosB ho ea haufi le tloaelo. Ha li kopantsoe hammoho, liphetho tsena li bonts'a hore liphetoho tse sa fetoheng ho dopaminergic neurotransication li hlahisa phetoho e tsoelang pele ea liproteine tse tšoanang le "deltaFosB" ka bobeli lithutong le maemong a holimo.
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