Ho lemoha liphetoho tsa limolek'hule ho methamphetamine-e sebelisitsoeng ho fos-expressing neurons e tsoang ho rat e le 'ngoe ea dorsal striatum e sebelisa ho hlophisa cell cell activated (FACS) (2013)

Methamphetamine le lithethefatsi tse ling li kenya karolo e nyane ea li-neuron tsohle bokong. Pele re ne re qapa mokhoa oa ho hlophisa lisele tsa fluorescence-activated cell (FACS) ho khetholla liphetoho tsa limolek'hule tse kentsoeng ka mokhoa o ikhethileng lits'ebetsong tsa li-neuron tse hlahisang letšoao la ts'ebetso ea neural Fos. Le ha ho le joalo, mokhoa ona o hloka sampole ea ho lata ho tsoa ho likhoto tse ngata. Ha joale re hlalosa mokhoa o fetotsoeng oa FACS o bonts'ang liphetoho liphatseng tsa limolek'hule tse tsoang ho Fos-expression dorsal striatal neurons ho tloha ho rat e le 'ngoe o sebelisa leano la makhetlo a mangata la ho ntlafatsa pele.

Fos and NeuN (letšoao la neuronal) immunohistochemistry e bonts'a hore 5-6% ea li-neurons tsa dorsal striatum li ile tsa qaptjoa 90 min kamora liente tsa methapo ea methamphetamine (5 mg / kg, ip) ha e le ka tlase ho 0.5% ea li-neuron li hlahisitsoe ka ente ea saline.

Re sebelisitse FACS ho arola li-neuron tsa NeuN tse ngotsoeng ho li-neuron tsa Fos-and Fos-negative mme ra hlahloba polelo ea mRNA re sebelisa RT-qPCR ho tsoa ho li-neuron tse hlano tse nang le Fos. Methamphetamine e kenyellelitse mefuta ea 3-20-fold e eketsehang ea liphatsa tsa pele tsa lefutso, li-Homer-2, c-fos, fosB, le isoforms tsa eona (ΔfosB le pale ea isoform ΔfosB-2) ka li-neurons tse ntle tsa Fos empa eseng Fos-negative. Induction ea gene mRNA ea pele-pele e ne e le tlase ka 10 kapa e le sieo ha ho hlahlojoa mehlala e sa tlosoang ho tsoa ho dorsal striatum homogenates. Mokhoa oa rona o fetotsoeng o etsa hore ho be bonolo ho ithuta liphetoho tse ikhethang tsa limolek'hule ka li-neuron tse hlahisoang ke lithethefatsi kapa litheko tse amanang le lithethefatsi mefuteng e rarahaneng ea ho lemalla.

Methamphetamine le lithethefatsi tse ling li kenya karolo e nyane ea li-neuron tsohle bokong. Mona re tlaleha mokhoa o ntlafalitsoeng oa ho bonts'a liphetoho tsa limolek'hule tse kentsoeng ka mokhoa o ikhethileng ho li-neuron tse hlahisang letšoao la ts'ebetso ea neural Fos. Re sebelisitse FACS mmoho le tokiso ea pele ea PCR e reretsoeng ho lekola polelo ea sethoathoa ea methapo ea methapo ea methapo ho tsoa ho ba mmalwa joaloka li-neurons tsa 5 Fos-expression tse tsoang ho rat e le 'ngoe ea dorsal striatum. Methamphetamine e hlohlelletsa 3-20-fold ho eketseha ha liphatsa tsa pele tsa lefutso (li-IEGs) ho li-Fos tse ntle empa eseng Fos-negative neurons. Phaello ea PCR e lebelletsoeng e etsa hore ho khonehe ho ithuta liphetoho tse ikhethang tsa limolek'hule ka li-neurons tse hlahisitsoeng ke lithethefatsi kapa litheko tse amanang le lithethefatsi mefuteng e rarahaneng ea ho lemalla.

E phatlalalitsoe 2013. Sengoliloeng sena ke mosebetsi oa 'Muso oa US ebile o sebakeng sa sechaba ho la USA.