Neuropharmacology. 2016 May 2. pii: S0028-3908 (16) 30185-X. Doi: 10.1016 / j.neuropharm.2016.04.045.
Wang J1, Bastle RM2, Bass CE3, Hammer RP Jr1, Neisewander JL2, Nikulina EM4.
inahaneloang
Ho imeloa kelellong ke sesosa se ka sehloohong sa ts'ebeliso e mpe ea lithethefatsi. Ho imeloa kelellong nakong e sa lekanyetsoang khatello ea kelello e eketsa ho itšireletsa lithethefatsi (SA) mme e phahamisa polelo e tsoang bokong e nkiloeng ke neurotrophic factor (BDNF) sebakeng sa ventral tegmental (VTA) ka likhoto. Intra-VTA BDNF overexpression e ntlafatsa ho hloloa ke khatello ea maikutlo e bakiloeng ke khatello ea maikutlo ho li-psychostimulants mme e baka polelo ea nucleus accumbens (NAc) ΔFosB. Ka hona, VTA BDNF e eketsehileng e ka etsisa kapa ea eketsa nts'etsopele ea boits'oaro bo amanang le ts'ebeliso e mpe ea lithethefatsi kamora khatello ea maikutlo ea sechaba. Ho leka khopolo-taba ena, kokoana-hloko e amanang le adeno (AAV) e kentsoe VTA ho hatella maikutlo ho feta GFP feela (taolo) kapa GFP + BDNF. Likhoto li ile tsa sebetsoa kapa tsa pepesetsoa khatello ea maikutlo ea sechaba pele li qala koetliso ea cocaine SA. Mekhahlelo ea SA ea ho fumana le ho e hlokomela e ile ea lateloa ke ho lekoa lenaneong le tsoelang pele la karolelano (PR) ea matlafatso ea koae, 'me nakong ea kopano ea "binge" ea cocaine ea 12-h. BDNF le ΔFosB li ile tsa hlalosoa ka mor'a lefu la 'mele libakeng tsa mesocorticolimbic circry li sebelisa immunohistochemistry. Ho imeloa kelellong khatello ea maikutlo ho ekelitse ts'ebeliso ea k'hok'heine lenaneong la PR, ho sa tsotelehe kalafo ea vaerase. Ha khatello ea maikutlo e le 'ngoe e eketsa ho kenella nakong ea boikoetliso ba li-12-h, likhoto tse hlotsoeng sechabeng tse amohetseng khatello ea maikutlo ea VTA BDNF li bonts'itse le ho feta ts'oaetso ea cocaine ha e bapisoa le sehlopha se hatelletsoeng ke GFP. Leha ho le joalo, khatello e fetelletseng ea VTA BDNF feela ha ea ka ea fetola ho itlopa joala. Polelo ea BDNF ho VTA le eona e ne e hokahane hantle le phepelo e felletseng ea cocaine nakong ea binge. Ho hatella ho feta ha VTA BDNF ho ekelitse polelo ea osBFosB ho NAc, empa eseng ho dorsal striatum. Mona re bontša hore khatello e fetelletseng ea VTA BDNF e eketsa tšebeliso ea k'hok'heine ea nako e telele, empa feela tlasa maemo a sithabetsang. Ka hona, polelo e ntlafalitsoeng ea VTA-BDNF e kanna ea ba mothusi bakeng sa keketseho e bakoang ke khatello ea maikutlo ea boits'oaro bo amanang le ts'ebeliso e mpe ea lithethefatsi haholo tlasa maemo a ts'oereng ts'ebeliso e mpe ea lithethefatsi.
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