J Vis Exp. 2015 Jan 20; (95). Doi: 10.3791 / 52329.
Tomas D.1, Pelejanto AH1, Ho phunya EL1, Hanna AJ1, Horne MK1, Tlhaloso ea Aumann TD2.
inahaneloang
Liphetoho tse tšoarellang bokong kapa 'polasetiki ea boko' li beha boits'oaro bo ntlafatsang le ho lokisa boko ka mor'a lefu kapa kotsi. Ntle le moo, ho sebelisana le tikoloho ea rona ho ka baka polasetiki ea boko. Ka mokhoa o ntseng o eketseha, lipatlisiso li leka ho supa hore na ke libaka life tse khothaletsang polasetiki ea boko e molemo bakeng sa ho phekola mathata a boko le boits'oaro.
Ho hlalositsoe mekhoa e 'meli ea tikoloho e eketsang kapa e fokotsa palo ea "tyrosine hydroxylase immunopositive" (TH +), e leng tekanyo ea "enzopamine" ea "dopamine (DA)"). Ea pele e na le ho kopanya litoeba tsa banna le tsa basali hammoho ka beke ea 1, e eketsang methapo ea methapo ea kutlo bohareng ba 12% ho ba batona, empa e fokotsa li-neurb tsa THB ka hoo e ka bang 12% ho basali.
Ea bobeli e na le litoeba tsa matlo tse tsoelang pele libeke tse 2 'libakeng tse ntlafalitsoeng' (EE) tse nang le mabili a mathang, lintho tsa ho bapala, liropo, lisebelisoa tsa sehlaha, jj.
Ntle le moo, protocol e hlalosoa e le hore ka nako e le 'ngoe e kenya lithethefatsi kahare ho bohareng nakong ea lits'ebetso tsena tsa tikoloho ho thusa ho tseba mekhoa e tlasa polasetiki ea boko e bakoang ke tikoloho. Mohlala, EE-induction ea li-midbrain tse ngata tsa TH + neurons e felisoa ke thibelo ea nako e le ngoe ea phallo ea synaptic ho li-neuron tsa midbrain. Hammoho, tlhaiso-leseling ena e bonts'a hore tlhaiso-leseling ka tikoloho e fetisoa ka ho kenya letsoho ho synaptic ho methapo ea methapo ea kutlo ho bulela kapa ho tima liphatsa tsa lefutso tsa 'DA'.
Ka hona, ts'usumetso e loketseng ea tikoloho, kapa sepheo sa lithethefatsi, se ka ba molemo bakeng sa ho phekola mathata a boko le boits'oaro a amanang le ho se leka-lekane ho midbrain DA (mohlala, lefu la Parkinson, khaello ea tlhokomelo le bothata ba ho se sebetse hantle, schizophrenia le ho lemalla lithethefatsi).
;
