https://doi.org/10.1038/s41386-019-0550-1
- Katherine F. Moore,
- Michael Z. Leonard,
- Nicholas M. Micovic,
- Klaus A. Miczek,
- Valentina Sabino &
- Pietro Cottone
Neuropsychopharmacology (2019) |
inahaneloang
Boitšoaro bo qobelloang ba ho ja bo khothalletsoa hore bo tsamaisoe ka karolo ke mefokolo ea moputso e ka bang ka lebaka la neuroadaptations tsamaisong ea mesolimbic dopamine (DA). Ka hona, sepheo sa thuto ena e ne e le ho lekola bofokoli tsamaisong ea meputso le tšebetso ea mesolimbic DA kamora ho feto-fetola chow e tloaelehileng le lijo tse monate, mohlala oa ho ja ka mokhoa o qobelloang. Mohlaleng ona, likhoto sehlopheng sa taolo (Chow / Chow) li fuoa lijo tse tloaelehileng matsatsi a 7 ka beke, ha sehlopha sa liteko (Chow / e khoneha) e fuoa chow bakeng sa matsatsi a 5 ka beke ("C Phase"), e lateloe ke matsatsi a 2 a phihlello ea lijo tse ka fumanehang habonolo ("Phase"). Pele re ile ra leka kutloisiso ea d-Tlhahiso ea Amphetamine e matlafatsang, e eketsang moputso, le melemo ea mantlha e sebelisang ts'ebetso ea ts'ebetso ea locomotor, ts'ebetso ea intracranial ea ho intlafatsa (ICSS), le tlhahlobo ea boemo ba boikhethelo, ka tatellano. Ka mor'a moo re ile ra hlalosa ho lokolloa ha DA ka mokokotlong oa li-nucleus accumbens (NAc) kamora kalafo le d-Amphetamine e sebelisa vivo micodialysis, tekanyetso ea "tyrosine hydroxylase" (TH) le transporter ea dopamine (DAT) mRNA e sebelisa bongata ba li-polymerase reaction reaction (qPCR), 'me qetellong, e lekantsoe le lintlha tsa motheo tsa extracellular DA le ts'ebetso ea DAT ka vivo e sebelisang "non-net". -flux ”microdialysis. Chow / e khoneha likhoto tse bontšitsoeng li hlabehile d-Amphetamine-ikiwaretse ts'ebetso ea locomotor, ho hloka matla ho d-Amphetamine potentiation ea ICSS monyako, le ho fokotsa khetho ea sebaka bakeng d-Amphetamine nakong ea Phase. Re fumane seo Chow / e khoneha likhoto li ne li tlotsitse tšebetso ea DA e latela d-Kalafo ea Amphetamine. Ho feta moo, DAT mRNA e ile ea eketseha ka Chow / e khoneha likhoto nakong ea Mokhahlelo oa Pele. Kamora nako, "microphalular" ea sen-no-flux, e senotsoeng, e senotse ts'ebetso ea motheo ea DA le letsatsi la DAT ho Chow / e khoneha likhoto. Ka kakaretso, liphetho tsena li fana ka bopaki ba ts'ebetso ea moputso e fokotsehileng e sebetsang le li-neuroadaptations tse amanang le litsamaiso tsa DA le DAT ka mokhoa ona oa ho ja ka mokhoa o phehellang. Khaello ea moputso, e hlahisoang ke ho ja ho tlola khafetsa, le eona e ka kenya letsoho ho ntšetsa pele boitšoaro bo qobelloang ba ho ja.
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