IKWU: Nnwale mbụ na-egosi na dopamine na-emetụta ọkwa DeltaFosb.
Ọnụ ego J Neurosci. 1996 Feb;8 (2):365-81 .
Doucet JP, Nakabeppu Y, Bedard PJ, Hope BT, Nestler EJ, Jasmin BJ, Chen JS, Iadarola MJ, St-Jean M, Wigle N, Blanchet P, Grondin R, Robertson GS.
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Ngalaba Nkà mmụta ọgwụ, Mahadum Ottawa, Ottawa, Ontario, Canada K1H 8M5.
nkịtị
N'iji ọgwụ mgbochi na-amata ngwaahịa nke ndị niile maara nke ezinụlọ fos nke mkpụrụ ndụ ihe nketa oge mbụ, e gosipụtara na mbibi nke ụzọ nigrostriatal site na 6-hydroxydopamine (6-OHDA) ọnya nke etiti ụbụrụ ụbụrụ na-emepụta ogologo oge (> 3). ọnwa) mbuli elu nke Fos-dị ka immunoreactivity na striatum. N'iji usoro nchọgharị traktị retrograde, anyị egosila na mbụ na mmụba a na Fos-dị ka immunoreactivity dị n'ọtụtụ ebe na neurons striatal na-arụ ọrụ na globus pallidus. N'ime ọmụmụ ihe dị ugbu a, a na-eme blọt Western na mwepụ nke nuklia sitere na striatum na-emebibeghị na nke 6-OHDA-lesioned iji chọpụta ọdịdị nke protein (s) Fos-immunoreactive na-akpata mmụba a. Ihe dị ka izu 6 mgbe ọnya 6-OHDA gasịrị, nkwupụta nke antigens abụọ metụtara Fos nwere ihe ngosi molecular nke 43 na 45 kDa na-eme ka ọ dịkwuo mma na striatum denervated. Nlekọta haloperidol na-adịghị ala ala na-ahọrọkwa okwu dị elu nke antigens metụtara Fos, na-atụ aro na ntinye ha mgbe nkwụsị nke dopaminergic na-eme ka ọ bụrụ onye na-eme ka ndị na-anabata dopamine D2 dị ka mbelata.
Western blot immunostaining iji mgbochi nke na-amata N-terminus nke FosB gosiri na 43 na 45 kDa Fos metụtara antigens nke dopaminergic denervation na nchịkwa haloperidol na-adịghị ala ala nwere ike jikọta ya na ụdị FosB gbajiri agbaji nke a maara dị ka deltaFosB. N'ikwekọ na atụmatụ a, nyocha nyocha retrograde gosipụtara na deltaFosB-dị ka immunoreactivity na deafferented striatum dị na striatopallidal neurons.
Nnwale ngbanwe nke gel gosipụtara na ọrụ AP-1 dị elu dị na denervated striata nwere protein (s) dị ka FosB, na-atụ aro na ọkwa deltaFosB kwalitere nwere ike mebie ụfọdụ mmetụta nke nkwụsị dopamine ogologo oge na mkpụrụ ndụ ihe nketa AP-1 na-achịkwa na neurons striatopallidal. N'ụzọ dị iche, nchịkwa na-adịghị ala ala nke D1-dị ka agonist agonist CY 208243 ruo 6-OHDA-lesioned oke kwalitere nke ukwuu deltaFosB-dị ka immunoreactivity na striatal neurons na-atụpụta na substantia nigra. Western blot immunostaining kpughere na deltaFosB na, ruo n'ókè dị nta, FosB na-ebuli elu site na nchịkwa D1 na-adịghị ala ala dị ka agonist. Ma mmeghachi omume reverse transcriptase-polymerase chain na nyocha nke ribonuclease gosipụtara na ọkwa deltafosB mRNA na-abawanye nke ọma na striatum denervated site na nchịkwa D1 na-adịghị ala ala.
N'ikpeazụ, anyị nyochara mmetụta nke nchịkwa na-adịghị ala ala nke D1-dị ka D2-dị ka dopamine agonists na okwu striatal deltaFosB na 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) ụdị mbụ nke ọrịa Parkinson. . N'ime enwe nke MPTP sụgharịrị Parkinsonian, enwere mmụba dị ntakịrị na protein (s) deltaFosB, ebe mmepe nke dyskinesia mepụtara site na nchịkwa D1 na-adịghị ala ala dị ka agonist na-esonyere nnukwu mmụba na protein (s) dị ka DeltaFosB. N'ụzọ dị iche, nchịkwa nke D2-dị ka agonist cabergoline na-eme ogologo oge, nke na-ebelata mgbaàmà Parkinsonian na-emepụtaghị dyskinesia na-ebelata ọkwa deltaFosB ka ọ dị nso. N'ịchịkọta ọnụ, nsonaazụ ndị a na-egosi na mgbanwe na-adịghị ala ala na dopaminergic neurotransmission na-emepụta mmụba na-adịgide adịgide nke protein (s) deltaFosB dị na ma rodent na primate striatum.
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