Na-egbu Biol. 2019 Feb 7. dị: 10.1111 / adb.12723.
Zhang T1, Yanagida J1, Kamii H.1,2, Wada S1, Domoto M1, Sasase H.1, Deyama S.1, Takarada T.1,3, Hinoi E1, Sakuru K4, Yamanaka A.5, Maejima T6, Mieda M.6, Sakurai T6,7, Nishitani N8, Nagayasu K8, Kaneko S8, Minami M2, Canada K1.
nkịtị
Na ọgwụ riri ahụ, mmegharị gburugburu ebe obibi nke metụtara cocaine na mbụ na-echeta ncheta metụtara cocaine, nke na-adịgide ogologo oge mgbe ịhapụsịrị ihe ma kpalite agụụ na ị coụ cocaine. Ọ bụ ezie na ọmụmụ gara aga na-atụ aro na medial prefrontal cortex (mPFC) na-etinye aka na ngosipụta nke cocaine-addictive omume, ọ ka edoghị anya ma ọ bụrụ na excitatory na inhibitory neurons na mPFC nwere ezi ihe metụtara otu na ncheta nke ncheta cocaine. Iji lebara okwu a anya, anyị ji ndị na-eme ihe nnabata ga-arụ ọrụ nke ukwuu site na teknụzụ ndị okike (DREADD) na-eji ntụpọ cocaine na-ebute ọnọdụ. Anyị kpuchiri ọrụ neeronal mPFC n'ụdị sel-yana oge. C57BL / 6J ụmụ oke ji ụmụ oke enweta mpekere intra-mPFC nke nje na-akpata adeno (AAV) na-egosipụta inhibitory DREADD (hM4Di) n'okpuru njikwa CaMKII onye na-akwalite onye na - ahọrọ oke iwepụ mPFC pyramidal neurons. GAD67-Cre mice nwetara ngbanye intra-mPFC nke AAV na-adabere na AAV na-ekwupụta hM4Di iji mezie GABAergic neurons nkịtị. Mgbachi Chemogenetic nke mPFC pyramidal neurons mere ka enwee ike inweta nnabata na nnabata nke CPP cocaine, ma iwelata MPFC GABAergic neurons emetụtaghị nnweta ma ọ bụ ngosipụta nke CPP cocaine. Ọzọkwa, mmachi nke chemogenetic nke mPFC glutamatergic neurons emetụtaghị nnweta na ngosipụta nke ọnọdụ ntụsara ahụ dị na lithium chloride. Nsonaazụ ndị a na-atụ aro na mmụba nke glutamatergic, mana ọ bụghị GABAergic, neurons na mPFC na-agbagha ma nhazi na iweghachite ncheta metụtara cocaine.
AMAOKWU: DREADD; mgbakwunye; koko; kẹle ẹhu-ụtso; cortex medial prefrontal; ebe nchekwa
PMID: 30734456
DOI: 10.1111 / adb.12723
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