Saini neural aberrant o le faia o faaiuga: Pathological gamblers fa'aalia cortico-striatal hypersensitivity i taaloga mata'utia.

• Sofie V. Gelskov^{a, b,,,,} ,
• Kristoffer H. Madsen^{a, c},
• Thomas Z. Ramsøy^{a, d, 1},
• Hartwig R. Siebner^{a, e, 1}

doi: 10.1016 / j.neuroimage.2016.01.002

  Tatala avanoa

faatumutumuga

• Pathological gamblers show U-shaped neural response to appetitive and aversive bets.
• This hypersensitivity is found in a cortico-striatal network, i.e. caudate and DLPFC.
• Sensitization of this network could constitute neural marker of compulsive gambling.
• A future focus on this network and action–outcome related mechanisms is proposed.

lē faʻatino

Pathological gambling is an addictive disorder characterized by an irresistible urge to gamble despite severe consequences. One of the hallmarks of pathological gambling is maladaptive and highly risky decision-making, which has been linked to dysregulation of reward-related brain regions such as the ventral striatum. However, previous studies have produced contradictory results regarding the implication of this network, revealing either hypo- or hypersensitivity to monetary gains and losses. One possible explanation is that the gambling brain might be misrepresenting the benefits and costs when weighting the potential outcomes, and not the gains and losses per se. To address this issue, we investigated whether pathological gambling is associated with abnormal brain activity during decisions that weight the utility of possible gains against possible losses. Pathological gamblers and healthy human subjects underwent functional magnetic resonance imaging while they accepted or rejected mixed gain/loss gambles with fifty–fifty chances of winning or losing. Contrary to healthy individuals, gamblers showed a U-shaped response profile reflecting hypersensitivity to the most appetitive and most aversive bets in an executive cortico-striatal network including the dorsolateral prefrontal cortex and caudate nucleus. This network is concerned with the evaluation of action–outcome contingencies, monitoring recent actions and anticipating their consequences. The dysregulation of this specific network, especially for extreme bets with large potentials consequences, offers a novel understanding of the neural basis of pathological gambling in terms of deficient associations between gambling actions and their financial impact.

uputatala

• Faia o faaiuga;
• Pathological taaloga faitupe;
• Cortico-striatal hypersensitivity;
• fMRI;
• Loss aversion;
• taui

faʻatomuaga

Pathological gambling is a mental disorder characterized by an irresistible urge to engage in monetary gambling despite harmful consequences. With a prevalence reaching 1–2% in many Western societies (Welte et al., 2008 ma Wardle et al., 2010), this disorder constitutes a severe public and personal health issue. Pathological gambling has recently been classified as a behavioral addiction and shares many core symptoms with drug addictions such as withdrawal, tolerance, and high preoccupation (Petry, 2007 ma Leeman ma Potenza, 2012).

Risky decision-making is an important hallmark of pathological gambling. Indeed, gamblers have a high tolerance toward risk (Clark, 2010 ma Brevers et al., 2013), and pathological gambling has been linked to alterations of dopaminergic regions linked to reward, risk, and motivation, such as the ventral striatum and the ventromedial prefrontal cortex (vmPFC) (van Holst et al., 2010, Limbrick-Oldfield et al., 2013 ma Potenza, 2014). However, while some studies have found hypoactivation of the mesolimbic reward pathway in response to the anticipation or outcome of rewards ( Reuter et al., 2005, de Ruiter et al., 2009 ma Balodis et al., 2012), other studies have reported hyperactivation of the same pathway to anticipated reward ( van Holst et al., 2012 ma Worhunsky et al., 2014), anticipated losses (Romanczuk-Seiferth et al. 2015), or gambling cues ( Crockford et al., 2005 ma Goudriaan et al., 2010). Interestingly, positron emission tomography (PET) studies revealed no general differences between gamblers and healthy controls in the magnitude of striatal dopamine release ( Joutsa et al., 2012 ma Linnet et al., 2011) but showed a positive correlation between striatal dopamine release and gambling severity (Joutsa et al. 2012), and dopamine release and gambling excitement (Linnet et al. 2011). These discrepant response patterns are reflected in two main accounts of pathological gambling. On the one hand, the reward deficiency theory predicts a hyposensitive reward system due to a dysfunctional dopamine D2 receptor found in substance addicts ( Blum et al., 1990 ma Noble et al., 1991) and gamblers ( Comings et al., 1996 ma Comings et al., 2001). A lower dopaminergic tone in the brain would push gamblers to seek higher rewards, in order to reach the threshold at which a “reward cascade” is initiated in the brain. On the other hand, the sensitization theory predicts a strong motivational bias toward objects of addiction ( Robinson ma Berridge, 1993 ma Robinson ma Berridge, 2008) leading to hypersensitivity in dopaminergic regions. In gamblers, the motivation to gamble would be triggered by gambling cues in the environment, which would override the incentive value of alternative sources of reward ( Goldstein ma Volkow, 2002 ma Goldstein et al., 2007).

These discrepancies underscore that the neural basis of pathological gambling remains unsettled. While studies contrasting monetary punishments and rewards can address how decision-values are computed in the brain, they do not address how gains and losses are integrated during gambling. Recently, we developed a gambling task that probes both the magnitudes of gain and loss values separately, as well as how gains and losses are balanced against each other in “mixed” (gain/loss) gambles (Gelskov et al. 2015). When balancing gains and losses, people tend to be more sensitive to potential losses than to equivalent gains, a decision-bias known as loss aversion (Kahneman and Tversky 1979). In practice, people typically reject 50/50 gambles unless they can win around twice as much as they can lose. Previous studies using mixed gambles with healthy participants found that the separate valuation of gains and losses involve reward-related dopaminergic target regions, specifically the ventral striatum and the vmPFC (Tom et al. 2007). However, when the entire gain/loss gamble is taken into account (i.e., potential gain, potential loss, and the consequences of winning or losing), other studies have found an important role for the amygdala in loss aversion (De Martino et al., 2010 ma Gelskov et al., 2015). In the present study, we used this task in a population suffering from gambling addiction as a means to gain insight into aberrant value-based decision-making.

Recently, a behavioral study found that problem gamblers are less loss averse than control subjects (Brevers et al. 2012, but see also Giorgetta et al. 2014). Here, we ask whether pathological gambling might reflect deficient balancing of possible gains against losses during decision-making. In a recent study, we found that activity of the amygdala and ventral striatum reflected the degree of loss aversion in healthy participants when they decided to accept or reject extreme gain–loss gambles (Gelskov et al. 2015). Here, we used individual gambling behavior to investigate how the decision-making process is tuned by inter-individual variation in loss aversion (i.e. being more or less loss averse), and whether loss aversion is also reflected in mesolimbic reward-related areas in gamblers. To address these issues, we used fMRI and a gambling task in which participants had to accept or reject mixed gambles on the basis of the ratio between the absolute gain and loss value. Our study design allowed us to address whether pathological gamblers balance positive and negative values differently from healthy controls and whether the integration of gain–loss ratios in gambling decisions is associated with abnormal activity in brain regions involved in value-based decision-making.

Mea ma metotia

Tagata e Auai

Fourteen male, un-medicated pathological gamblers (mean age in years: 29.43; SD: 6.05; range: 20–40) and 15 healthy control subjects (all male; mean age in years: 29.87; SD: 6.06; range: 21–38) were recruited specifically for this study. Two additional gamblers were initially scanned but excluded before inclusion in the analysis because they misunderstood the task: One participant only responded when accepting a bet, while another participant thought that all gambles would be paid out at the end of the session. Gamblers were recruited through a Danish treatment center for pathological gambling. No participant had additional mental health issues apart from pathological gambling based on the structural clinical interview for DSM-IV, Axis I (SCID-I, Research version, patient and nonpatient versions; First et al. 2002), including disorders such as drug use or dependency. The presence of pathological gambling was confirmed by structural interview based on the SCID module for pathological gambling. All gamblers had a South Oaks Gambling Screen (SOGS) score above 5 (Laulau 1; Lesieur and Blume 1987; Danish versions of SOGS and SCID modules were translated by J. Linnet). Participants were screened for MR compatibility, history of neurological disorders, and signed informed consent forms. The study was approved under the ethical protocol KF 01–131/03, issued by the local ethics committee.

Laulau 1.

Demographic and neuropsychological characteristics of participants.

Abbreviations: SOGS, South Oaks Gambling Screen; AUDIT, Alcohol Use Disorders Identification Test; WAIS, Wechsler Adult Intelligence Scale; BDI, Beck Depression Inventory; BIS-11, Barratt Impulsiveness Scale, 11th ed., GAD-10, Generalized Anxiety Disorder test; DOSPERT, Domain-Specific Risk-Taking scale.

a

Highest educational level (scoring): 1 = Lower/general secondary school, 2 = vocational education and training, 3 = upper secondary school, 4 = professional college degree, 5 = bachelors degree or similar, 6 = masters degree.

b

One gambler did not complete the AUDIT screen, one did not complete the smoking and educational screen. One control subject did not complete the BIS-11 questionnaire.

c

Non-parametric permutation test used due to non-normal distributions.

Filifiliga o le laulau

Participants were tested on two separate days 1–2 weeks apart. During the first test session, participants underwent neuropsychological testing, questionnaires, and interviews (see Laulau 1). Participants were also endowed with 200 Danish Kroner (i.e., the Danish monetary currency, DKK, 1 DKK ≈ 0.16 US dollar), which they were told to bring back the following week for the fMRI test session as a gambling stake.

Gambling task and stimuli

During the fMRI session, participants performed a gambling task, which required them to accept or reject mixed gain–loss gambles with equal probability of winning or losing (Mati. 1A). On each trial, subjects were presented with a pie chart with either a potential gain amount or a potential loss amount, according to main condition (i.e. “loss first” or “gain first” conditions). After a varying display time (2–5 s), the second amount of the mixed gamble was presented and subjects decided to accept or reject the current gamble by pressing one of two buttons in the scanner. Both, the first “magnitude presentation phase” and the ensuing “decision phase” were jittered in steps of 0.5 s (i.e., 2, 2.5, 3, 3.5, 4, 4.5, and 5 s) pseudo-randomly from trial to trial. Instructions were read aloud to the participants, where after they completed a short training session until they were familiar with the task. Participants were told that no feedback would be given about the outcome of single bets during scanning, but that after the fMRI session, the computer would select two random bets: the ones that had been taliaina during the gambling session, would be “played out” and participants would either lose money from their endowment or win additional money, while if they had rejected the bet, no 50/50 gamble was played out. Participants were told to follow their “gut feelings” and that there was no right or wrong answers.

Fig. 1. 

Gambling task in the scanner, stimulus matrix, and choice behavior. A) Event-related fMRI paradigm; participants first received either a potential loss or a potential gain amount (i.e. the magnitude “Presentation” phase). Then, when both amounts were presented, participants chose whether to accept or reject the gamble (i.e., “Decision” phase). Inter-trial intervals (ITIs) separated trials. NB: “kr” = “DKK”. B) Color-coded heat map representing gambling ratios (gain/loss). Stimuli consisted of 64 different gain–loss ratios, corresponding to 8 potential gain amounts (68–166 DKK; increments of 14) by 8 potential loss amounts (34–83 DKK; increments of 7). Color-coding reflects ratios from lowest (0.82) to highest (4.9). All gain/loss ratios were presented twice in randomized order, once in a “gain first” and once in a “loss first” condition. C) Color-coded heat maps representing choice patterns for gamblers (left) and controls (right). The color-coding from black to red to yellow to white reflects the increasing percentage of accepted gambles (black ➔ white: 0–100%). D) Loss aversion coefficient, lambda (λ), for all participants. Note the right skewed distribution. Non-parametric permutation test indicated a trend toward less loss aversion in pathological gamblers compared to healthy controls (P = 0.077).

Filifiliga ata

Stimuli consisted of mixed gambles presented on yellow and purple pie charts with one monetary amount (i.e., potential gains and losses in Danish currency) presented in each half of the chart (Mati. 1A). The 64 stimuli combined the 8 potential gain amounts (68–166 DKK; in increments of 14 DKK), with the 8 potential loss amounts (34–83 DKK; in increments of 7 DKK; see gain/loss ratio matrix in Mati. 1B). The 64 mixed gambles were presented once in a “gain first” and once in a “loss first” condition, yielding a total of 128 trials. Each of the stimuli belonged to one of 8 classes, identified by the angle of the pie chart which was rotated with 45° (0°–360°) for each class. Thus, although each amount (e.g. + 82 DKK) appeared 16 times, it was only presented once in the same physical position on the screen per main condition (gain or loss first), as to avoid any low-level repetition effects. To ensure that subjects were attentive to the task and to increase the amount of ratios below 1, we added 18 highly disadvantageous catch trials. These trials combined 3 low-gain amounts (i.e., 34, 41, 48 DKK) with 3 high-loss amounts (i.e., 138, 152, 166 DKK). All subjects rejected at least 89% of the catch trials, indicating that subjects paid attention to the task (gamblers rejected 98% of all catch trials; range: 95–100%; control subjects rejected 98.9% of catch trials; range 89–100%). There was no difference in proportion of rejected catch trials between groups (P = 0.61, t(27) = 0.52, SD = 2.99). Finally, we added 24 “baseline” trials: empty pie charts without any amounts (note that neither catch trials nor baseline trials were used in the behavioral analysis or included as regressors of interest). Stimuli were presented and button presses recorded using the E-Prime 2.0 software (Psychology Software Tools, Pittsburgh, PA).

Based on the participant’s choices on the 128 regular trials, we calculated the individual degree of loss aversion, lambda (λ), by fitting a logistic regression to each participant’s binary response (accept/reject). In contrast to Tom et al. (2007), we used the full gain/loss ratio of the mixed gambles as independent variable to derive the individual “decision-boundary” lambda in each participant. This was due to our focus on the full gamble ratio in the fMRI analyses, rather than the single gain and loss values. Lambda was estimated as the gain/loss ratio for which the probability of accepting a trial was equal to the probability of not accepting a trial (i.e. 0.5).

Faʻaauau foliga faʻapitoa

Functional and structural brain scans were acquired using a Siemens Magnetom Trio 3 T MRI scanner with an 8-channel head coil. Blood oxygen level dependent (BOLD) functional MRI was collected using a T2*-weighted echo-planar imaging sequence (295 volumes; 41 slices; 3 mm isotropic resolution; repetition time: 2430 ms; echo time: 30 ms; flip angle: 90°; field of view: 192 mm, horizontal plane) optimized for detecting BOLD signal in the orbitofrontal cortex (Deichmann et al. 2003). Slices were oriented axially and the phase encoding direction was anterior–posterior. Note that the orientation of the field of view did not allow full coverage of the superior parietal cortex. A high-resolution three-dimensional structural scan of the whole brain was acquired using a T1-weighted magnetization prepared rapid acquisition gradient echo (MPRAGE) sequence for the purpose of manual co-registration (1 mm isotropic voxels; FOV: 256 mm; acquisition matrix 256 × 256; TR: 1540; TE: 3.93 ms, inversion time: 800 ms, and a flip angle of 9°) and creating a group-specific normalized anatomical template for display of functional maps in the figures. The first two volumes were discarded as dummy scans to allow the field to reach steady state.

Iloiloga o faʻamatalaga fMRI

The fMRI data were analyzed using SPM8 software (Wellcome Department of Cognitive Neurology). Pre-processing included slice time correction, spatial realignment to the mean image, manual co-registration of images, normalization to a standard EPI image (i.e., MNI template image; functional voxels of 2 × 2 × 2 mm), smoothing using an isotropic 8 mm full-width at half maximum Gaussian kernel, and high-pass temporal filtering (cut-off frequency 1/128 Hz). The general linear model (GLM) estimated a 24-parameter Volterra expansion of the 6 estimated motion rigid body realignment parameters, which were included as regressors of no interest as described in Friston et al. (1996). We also included additional regressors for catch trials, error trials (i.e., 250 ms > reaction time > 2500 ms and trials with no answer) as well as two “button-press regressors” modeling out the motor activation related to finger button presses. In five subjects, brain volumes were excluded because of excessive head movement (i.e., global head movement above 8 mm, local head movement above 2 mm), and DVARS (i.e., the root mean squared (RMS) change in BOLD signal from volume to volume, where «D» refers to the temporal derivative of time courses and «VARS» to the RMS variance over voxels above 5% change in global BOLD signal as defined in Power et al., (2012)).

In each participant, we captured task-related BOLD signal changes using a GLM, which modeled the magnitude presentation phase and decision phase of each trial (see Mati. 1A). The BOLD signal changes during the magnitude presentation phase was divided into separate “gain events” and “loss events,” each modeled with their individual amounts as parametric linear modulations. BOLD signal changes during decision-making were parametrically modulated with the absolute gain–loss ratio including a first (i.e. linear) and second (i.e. quadratic) order polynomial modulation (i.e (gain/loss)²). All regressors of interest were convolved with the canonical hemodynamic response function.

The individual parameter estimates for first and second order polynomial modulation of increasing gain–loss ratios was then entered in two separate second-level group analyses. These second-level t-tests included the individual loss aversion score (i.e., lambda) as covariate to model the influence of individual differences in loss aversion. A separate second-level model included the individual SOGS scores as index of gambling severity. Differences in regional BOLD response between gamblers and controls were assessed using two-sample t-test. At the group level, clusters were considered significant if they exceed a threshold of P < 0.05 corrected for multiple comparisons with family-wise error correction across the whole brain (i.e. on a cluster level), using an entry threshold of P_{Le faasa'oina} < 0.001. In addition, various trend activations in relevant cortico-limbic structures are reported at P_{Le faasa'oina} < 0.001. Coordinates are displayed in MNI stereotactic space. For the purpose of highlighting the main BOLD activation clusters (i.e. caudate and DLPFC, Mati. 4) and performing scatter plots of parameter estimates based on individual behaviors (i.e. plotting loss aversion in amygdala and gambling severity in precuneus, Mati. 5), we created anatomical masks for these regions using the WFU PickAtlas (Maldjian et al. 2003). For the masks covering bilateral caudate, amygdala, and precuneus, we used predefined “AAL” atlas masks (Tzourio-Mazoyer et al. 2002), while for the DLPFC mask, we constructed a mask covering Brodmann areas 8–10, 46, and the middle frontal gyrus (MFG). Note that none of these masks were used to ameliorate any of the fMRI results reported in the main text or in the tables.

i'uga

Demographic and neuropsychological data

Demographic and neuropsychological data are listed in Laulau 1. Groups did not differ significantly with respect to age, handedness, general anxiety, or alcohol dependency. However, gamblers showed slightly higher smoking dependency, lower educational level, higher overall impulsiveness and differed in the way they perceived risks compared to non-gambling controls. Importantly, all gamblers had a SOGS of more than 5, indicating that they were all in the pathological range (median: 10; range: 6–19). In contrast, all but two control subjects scored 0 on the same test (median: 0; range: 0–3), indicating no problems with gambling.

Depression is a common co-morbidity in pathological gamblers, and consistently, we also found a substantial increase in depressive symptoms in the gambling group compared to the control group. However, there was no correlation between gambling behavior (i.e., λ) and BDI scores in the gamblers (R = 0.2739, P = 0.3651).

We also found a significant difference in performance on the WAIS subtests probing vocabulary and general knowledge (“information”) levels. Again, we found no correlations between these measures and gambling behavior (i.e., correlation between WAIS information and λ: R = 0.0124, P = 0.9679; and between WAIS vocabulary and λ: R = 0.2320, P = 0.4456).

Fa'amatalaga tau amio

Mati. 1C shows the distribution of accepted gambles for a given gain–loss ratio for gamblers and controls. Most participants consistently showed loss averse behavior: They accepted a given gamble only when the gain amount clearly exceeded the loss amount (i.e. lambda > 1). Gamblers tended to be less loss averse. The mean proportion of accepted vs. rejected trials in gamblers was 65% vs. 35%, and in controls, 55% vs. 45%, but inter-individual variability was substantial in both groups: median lambda in gamblers was 1.45 (SD = 0.49; mean = 1.45; range: 0.56–2.59), with a positively skewed distribution of λ’s (skewness coefficient of 0.42), while median lambda in healthy controls was 1.82 (SD = 0.83; mean = 1.83; range: 1.01–3.83; positive skewness: 0.93). Therefore, the difference in lambda between groups only reached borderline significance (P = 0.077; t(27) = 1.47). Note that the lambda distribution was non-normal (Shapiro–Wilks test of normality: P = 0.0353, W = 0.9218). We therefore employed a random permutation test based on resampling (also known as a randomization test) to assess differences in lambda between pathological gamblers and healthy controls. The number of iterations used was 10.000.

The number of error trials was comparable between groups. Gamblers as a group had 30 error trials (15 non-response, 15 very fast or slow responses) with 0–8 error trials per subject. Control subjects made in total 27 errors (16 non-response, 11 very fast or slow responses) with 0–8 error trials per subject. Mean response times were also similar between groups (P = 0.66; t(27) = 0.45; gamblers: 927 ms; SD = 240; controls: 959 ms; SD = 122). Decisions to accept or reject a gamble were more difficult when the subjective utility of gains and losses were similar. This was reflected in response times, as both groups responded slower when the Euclidian distance between the individual gain/loss ratio and the group mean lambda decreased (gamblers: R = 0.15, P < 0.001; controls: R = 0.15, P <0.001).

Linear increase in neural activity with increasing gain–loss ratios

In the decision-making phase, a large bilateral cluster in the anterior cingulate cortex (ACC) and the vmPFC (P < 0.001; x, y, z = − 8, 40, 6; Z = 4.75; k = 759), bilateral mid-cingular cortex and adjacent precuneus, (P < 0.001; x, y, z = − 10, − 30, 52; Z = 4.43; k = 1933), and superior frontal gyrus (SFG; P < 0.001; x, y, z = 18, 38, 56; Z = 4.34; k = 633) showed a linear increase in BOLD response with increasingly appetitive gain–loss ratios across all 29 participants. Mati. 2 shows that this linear effect was mainly driven by the gamblers, who showed a gradual increase of the BOLD response with increasingly appetitive gamble ratios in the pregenual portion of ACC (P < 0.001; x, y, z = − 8, 36, 8; Z = 5.18; k = 518; Mati. 2A) and the right vmPFC (P = 0.003; x, y, z = 8, 34, − 10; Z = 4.23; k = 307) as well as in the mid cingulum/precuneus (P = 0.031; x, y, z = − 10, − 30, 52; Z = 4.40; k = 188), right inferior temporal gyrus/parahippocampus (P = 0.002; x, y, z = 34, 2, − 30; Z = 4.23; k = 329), and postcentral gyrus (P = 0.001; x, y, z = 62; − 20, 44; Z = 4.11; k = 356). Control subjects, on the other hand, showed dispersed activation clusters in a range of areas (left precuneus: P < 0.001; x, y, z = − 6, − 58, 32; Z = 4.72; k = 1010; right lingual gyrus: P = 0.002; x, y, z = 18; − 86, − 8; Z = 4.67; k = 332; left cuneus: P = 0.028; x, y, z = − 14, − 100, 10; Z = 4.27; k = 193; and right posterior lobe of the cerebellum: P = 0.001; x, y, z = 42, − 70, − 34; Z = 4.09; k = 351) with peak activation in the left angular gyrus (P < 0.001; x, y, z = − 48, − 60, 30; Z = 5.06; k = 433; Mati. 2B). Although we found no significant decreases in activation for increasingly appetitive bets, we did find trends in the anterior insula of the control group (L: P < 0.001, uncorrected; x, y, z = − 32, 24, − 2; Z = 3.83; k = 74; R: P < 0.001, uncorrected; x, y, z = 42, 24, 4; Z = 3.64; k = 14). When contrasting the groups, no significant differences were found. However, gamblers showed a trend toward a higher increase in activity with increasingly appetitive gambles in the left pregenual ACC (P < 0.001, uncorrected; x, y, z = − 8, 36, 6; Z = 4.33; k = 98; Mati. 2C). Results showing the impact of individual degree of loss aversion on the linear increase in neural activity with increasing ratios can be found in Supplementary Fig. 1 and Supplementary Table 1.

Fig. 2. 

Color-coded statistical t-score maps: Brain regions showing a positive linear relationship between the BOLD response and increasing gain–loss ratios of the gambles A) in gamblers, B) in controls, and C) contrasting the two groups. When contrasting groups, BOLD activation revealed a trend difference in pregenual ACC (gamblers > controls). Maps are thresholded at P < 0.001 (uncorrected) and displayed on a group-specific normalized anatomical template based on structural T1 images.

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Quadratic increase in neural activity with increasing gain–loss ratios

When combining BOLD signal from all participants, a large network of prefrontal areas in the dorsal and mesial frontal lobe showed a quadratic increase in neural activity with increasing gain–loss ratios peaking in right dorsal SFG (P < 0.001; x, y, z = 12, 24, 60; Z = 5.38; k = 1769). Further activations for this contrast included the left middle frontal gyrus (P < 0.001; x, y, z = − 38, 10, 50; Z = 4.81; k = 605), bilateral angular gyri (L: P = 0.022; x, y, z = − 42, − 64, 40; Z = 4.24; k = 227; R: P < 0.001; x, y, z = 52, − 56, 38; Z = 4.68; k = 488), left inferior frontal gyrus (P = 0.004; x, y, z = − 42, 26, − 16; Z = 4.09; k = 330), and right inferior temporal gyrus (P = 0.001; x, y, z = 66, − 14, − 22; Z = 4.30; k = 409). As shown in Mati. 3, separate analyses for each group reveal that this effect was only consistent in gamblers. In gamblers, several brain areas showed quadratic increases as a function of gamble ratios, including a large bilateral prefrontal cluster covering the dorso-lateral parts of middle and superior frontal gyri, and a focalized subcortical cluster covering head and body of both left and right caudate nuclei (Mati. 3A; full list of activations can be found in Laulau 2). In contrast, the activity profile in controls did not reflect any quadratic modulation of activity with increasing gain–loss ratio (Mati. 3B; Laulau 2).

Fig. 3. 

Color-coded statistical t-score maps: Brain regions showing a positive quadratic relationship between the BOLD response and increasing gain–loss ratios of the gambles in A) gamblers, B) controls, and C) contrasting the two groups. Maps are thresholded at P < 0.001 (uncorrected).

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Laulau 2.

Functional MRI results: quadratic increases in regional BOLD activity with increasing gamble ratios.

P < 0.05, FWE corrected at cluster level.

⁎

Local maxima within cluster with Z score > 4.

Filifiliga o le laulau

When contrasting gamblers with controls, we found significantly stronger quadratic modulation of neural activity with gain–loss ratio in a large set of brain regions (Mati. 3C), including the large bilateral cortico-striatal cluster. Within this cluster, the left caudate nucleus showed the strongest group difference at the subcortical level and the right DLPFC displayed the strongest group effect at the cortical level. The full list of activation clusters is given in Laulau 2. Noteworthy, no clusters displayed stronger quadratic modulation of neural activity with gain–loss ratio in controls compared to gamblers.

It should also be noted that the quadratic BOLD increase to aversive and appetitive gambles survived in gamblers even when including BDI or the WAIS scores as covariates in the second-level t-tests (i.e. modeling out the effect of depression, vocabulary or general knowledge levels, which were differing between groups according to behavioral tests, see Laulau 1). Results, where the effect of depression has been modeled out of the quadratic increase in neural activity with increasing ratios, can be found in Supplementary Fig. 2.

To illustrate the underlying shape of the quadratic modulation of BOLD signal during decision-making, we assigned each of the 64 gain–loss ratios to one of 16 adjacent”bins” in a post hoc GLM. When plotting activation in each of these bins as a function of increasing gain–loss ratio, we found that the BOLD response profile in gamblers was U-shaped (Mati. 4B). In order to determine if a linear or a cubic model was more appropriate to describe the effect, we tested if the additional variance explained by including higher order polynomial terms (quadratic and cubic) were significant. In gamblers but not controls, a nested regression model verified that the quadratic fit was more appropriate to describe the nature of the curve, than a linear fit. Note that these descriptive data should not be seen as separate results, but merely a complementary analysis to illustrate the underlying shape of the BOLD response profiles.

Fig. 4. 

U-shaped modulation of the BOLD response to increasing gain–loss ratios in pathological gamblers. A) Color-coded statistical parametric maps showing clusters with higher sensitivity to extreme positive and negative gain–loss ratios in gamblers compared with controls. Maps are thresholded at P < 0.001 uncorrected. To highlight the two principal regions differing between groups, anatomical masking of the caudate nuclei (top) and DLPFC (bottom) is used. B) These scatter plots are based on a “post hoc” GLM analysis created for illustrative purposes, where adjacent gain–loss ratios were clustered together into 16 ratio-“bins” (the range of ratios is displayed on the x-axis). The y-axis indicates regional neural activity (as estimated by the BOLD response in an 8-voxel sphere around peak activation) in the decision phase for gamblers (red) and controls (black). A nested regression model suggest that activation is better explained by a quadratic compared to a linear relationship with gain–loss ratio in the caudate nucleus (P = 0.02) and DLPFC (P = 0.02) in gamblers (left panel) but not in controls (right panel).

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Impact of individual loss aversion

Across both groups, the individual degree of loss aversion, indexed by the individual decision-boundary lambda, enhanced the sensitivity to extreme gain–loss ratios of mixed gambles in a network of brain regions with peak activation in right amygdala (P < 0.001; x, y, z = 24, − 4, − 26; Z = 5.01; k = 1988). Apart from the main activation peak in the amygdala, regions included the DLPFC/SFG (P < 0.001; x, y, z = 32, 24, 56; Z = 4.86; k = 2372), left middle temporal/parahippocampal gyrus (P < 0.001; x, y, z = − 44, − 24, − 24; Z = 4.59; k = 1435), precuneus (P < 0.001; x, y, z = − 4, − 62, 26; Z = 4.40; k = 1169), and vmPFC (P = 0.009; x, y, z = 8, 26, − 18; Z = 4.31; k = 281).

In pathological gamblers, the individual degree of loss aversion was associated with an enhanced sensitivity to extreme gain–loss ratios in a dorsal frontal network with a regional peak in the DLPFC (Mati. 5A; see also Laulau 3 for full list of activations). This cortical network closely resembled the prefrontal areas showing a U-shaped activity increase with increasing gain–loss ratios in gamblers presented in Mati. 3.

Fig. 5. 

Modulation of the U-shaped relationship between neural activity and gain–loss ratios by A) individual degree of loss aversion and B) severity of gambling. A) Color-coded statistical parametric maps illustrating how degree of individual loss aversion (reflected by high individual λ-values) enhanced the U-shaped relationship between neural activity and gamble ratios in pathological gamblers (left panels) or controls (right panels). The below graph illustrates the relation between the individual parameter estimate for the U-shaped relationship between neural activity and gain–loss ratios (y-axis) and individual loss aversion (x-axis) in the bilateral amygdala (controls: P <0.001; R² = 0.83; gamblers: P = 0.11; R² = 0.71). B) Top: Color-coded statistical parametric map showing a bilateral cluster in precuneus, where the neural sensitivity to extreme gambles increased with gambling severity in pathological gamblers. Right: The scatter plot shows the linear relation (P = 0.016; R² = 0.63) between individual parameter estimates of the U-shaped relation between ratio and neural activity in the precuneus region (y-axis) and the individual gambling severity expressed by individual SOGS scores (x-axis). All BOLD activations are whole brain activations displayed at threshold P < 0.001 (uncorrected).

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Laulau 3.

Functional MRI results: effect of loss aversion on quadratic increase in regional BOLD activity with gamble ratios.

P < 0.05, FWE corrected at cluster level.

⁎

Local maxima within cluster with Z score > 4.

Filifiliga o le laulau

In non-gambling controls, a more ventral and posterior network showed enhanced sensitivity to extreme gamble ratios as a function of loss aversion, with the right amygdala having the strongest effect size (Mati. 5A, middle right panel; Laulau 3). The direct comparison of the two groups yielded a significantly stronger effect of loss aversion on the activity profile in the DLPFC for gamblers compared to controls (Laulau 3), whereas the modulatory effect of loss aversion on amygdala activity was not significantly different between groups.

When plotting the relationship between BOLD parameter estimates and loss aversion, individual loss aversion in the healthy controls (but not gamblers) enhanced the U-shaped relationship between neural activity in the amygdala (Mati. 5A, bottom graph. Note that this effect was robust to the exclusion of the most loss averse control subject). With the exception of a few voxels in the right amygdala (see Mati. 5A, middle panel), loss aversion in pathological gamblers was not linked to altered amygdala response during decision-making.

Impact of severity of pathological gambling

We investigated whether gambling severity in gamblers as indexed by the individual SOGS scores modified the U-shaped response to extreme ratios during decision-making. A whole-brain search revealed a focal enhancement of the sensitivity to extreme ratios with gambling severity in bilateral precuneus (P = 0.003; x, y, z = − 6, − 48, 40; Z = 4.59; k = 335; Mati. 5B, top panel). Accordingly, the correlation between percent BOLD signal changes in a bilateral precuneus region (restricting activity to this region through anatomical masking) and gambling severity was highly significant (Mati. 5B, bottom graph).

Brain responses to single potential gains and losses

Since the win and loss amount of a mixed gamble was presented sequentially in each trial, we were able to capture regional changes in the BOLD signal corresponding to single potential gains and losses (but see also discussion of the jittering used in the Talanoaga section). During this passive evaluation phase, we searched for between-group differences in BOLD response to gains, losses, increasing gains, and increasing losses. There were no significant group differences for these contrasts, but we found a bilateral trend toward a higher BOLD response to potential gains in gamblers compared to controls in the amygdala (L: P < 0.001, uncorrected; x, y, z = − 26, 2, − 22; Z = 3.19, k = 6; R: P < 0.001, uncorrected; x, y, z = 24, − 2, − 10; Z = 3.43; k = 7).

Talanoaga

Contrasting healthy and pathological decision-making with a mixed gamble task, we measured task-related neural activity during gambling decisions, which required participants to trade off a possible gain against a possible loss. In gamblers, a dorsal cortico-striatal network displayed a higher neural sensitivity to the most appetitive and aversive gain–loss ratios compared to healthy matched controls. The stronger tuning of dorsal cortico-striatal areas to extreme gain–loss ratios indicates that gamblers put more weight on the extremes of the decision frame offered by the gambling task. Importantly, this U-shaped neural response to gambling ratios was not observed in controls, suggesting that this specific hypersensitivity to extreme ratios constitutes a neural signature of pathological gambling.

Interestingly, the U-shaped tuning of neural activity to the most aversive and most appetitive gambles was not expressed in core regions of the reward network, such as ventral striatum or orbitofrontal cortex. Instead, it was expressed bilaterally in a dorsal cortico-striatal “associative” or “executive” network, including the caudate nucleus and the DLPFC. The recruited DLPFC included the dorsal and mesial superior and middle frontal gyri, corresponding to BA 6/8/9 and “9/46d” (Badre and D’Esposito, 2009 ma Goldstein ma Volkow, 2011). This dorsal cortico-striatal network is known to be involved in monitoring recent actions and anticipating their outcomes (for review see Yin and Knowlton 2006). In particular, the human caudate nucleus has been implicated in the reinforcement of action–outcome contingencies (Knutson et al., 2001, O'Doherty et al., 2004, Tricomi et al., 2004 ma Delgado et al., 2005).

Our present results suggest that this dorsal cortico-striatal network plays an important role in gambling decisions made by gamblers. Extreme gain–loss ratios are characterized as being highly relevant in terms of possible action–outcomes: the more appetitive a bet is, the more important it is to accept it; conversely, the more aversive a bet is, the more important it is to reject it. In healthy subjects, the dorsal striatum has been found to track stimulus salience or arousal, rather than linearly increasing subjective value (Barta et al. 2013). We infer that in pathological gamblers, this dorsal cortico-striatal network is hypersensitive and weights these extreme gain–loss ratios more strongly than in healthy subjects, when making gambling decisions.

Current theories of the neurobiological bases of pathological gambling are compelling in their simplicity, by predicting either a hypo- or hypersensitivity of the ventral striatum and other ventral core regions of the reward system such as the vmPFC. Accordingly, previous neuroimaging studies in gamblers showed either diminished (Balodis et al. 2012) pe faʻaleleia (van Holst et al., 2012 ma Worhunsky et al., 2014) activation of ventral striatum during the anticipation of monetary reward. In the present study, no differences in neural activity between pathological gamblers and non-gambling controls emerged in the ventral reward system when they evaluated single loss or gain amounts during the magnitude presentation phase or when they balanced possible gains and losses of the mixed gambles in the decision phase. Only the right and left amygdala showed a trend toward a stronger neural response to possible gains during the former phase. In other words, the decision to accept or reject a gamble was not consistently associated with a hyper- or hyposensitivity of the reward system. This negative finding is in agreement with a recent study where gamblers showed a normal reactivity of the ventral striatum to monetary reward cues but a blunted sensitivity to cues predicting erotic stimuli (Sescousse et al. 2013). The lack of a consistent pattern within this literature, with basically either opposite results or no striatal effect at all, indicate that explaining pathological gambling by striatal up- or down-regulation might not be adequate. It has been suggested that the decision-making deficits seen in pathological gambling could emerge from an imbalance between dopaminergic systems involving limbic motivational structures and prefrontal control regions, rather than a disruption in either component in isolation (Clark et al. 2013). One good candidate of such cortico-striatal networks is the dorsal cortico-striatal loop, which has been implicated in action selection and processing action outcome contingencies (Yin ma Knowlton, 2006 ma Seo et al., 2012). Note that in the present study decisions are made based on internal representations of the balance between gains and losses rather than on outcome-based adaptive processes, or strictly anticipatory processes. This is perhaps the reason why we find areas that are more related to the choice of the action (i.e. accepting or rejecting a bet), rather than areas traditionally coding for anticipating or receiving outcomes.

Here, in non-gambling controls, loss averse behavior during the gambling task was associated with a stronger sensitivity to extreme gain–loss ratios in the amygdala. These results correspond well with our recent findings in a separate group of healthy individuals (Gelskov et al. 2015), where more loss averse participants showed an increased neural sensitivity in the amygdala to extreme gain–loss ratios of mixed gambles. These results persisted despite subtle differences between studies. The actual game participants played in the scanner remained the same (i.e. distribution of monetary amounts, duration, and jittering of visual stimuli, etc.). However, the endowment procedure differed slightly. In the current study, participants received actual money bills (200 DKK) that they kept for 1–2 weeks before entering them as a stake in the gamble, while in the previous study, participants were led to believe they could lose money from their initial endowment. This difference in endowment strategy could perhaps explain why the healthy control subjects in the present study were a bit less loss averse (median lambda of 1.82) compared to our previous study (median lambda of 2.08). Although the statistical difference between the two healthy groups was not significant (P = 0.18, permutation test), the difference in lambda between the previous healthy group and the current group of gamblers was significant (P = 0.004, permutation test). Another obvious difference between the studies is the age difference, as the present control group was older in order to match the gamblers (P = 0.0175, t(29) = 2.52; 2-sample t-test). However, if anything, this difference should predict the opposite effect on lambda, since older healthy subjects tend to be more loss averse than younger. Furthermore, the two studies differed slightly in the way gamble ratios were modeled. In our previous study, we found that the amygdala was sensitive to variations in gain–loss ratios in relation to a subject-specific “decision-boundary” (i.e. the individual lambda score, λ). This model can be conceptualized as “V”-shaped BOLD response to increasing ratio, where the “low point” of the V was the individual λ-score. Two linear parametric regressors then classified each trial ratio as being more or less appetitive or aversive, according to how they differed from the individual λ (i.e., aversive ratios < individual λ < appetitive ratios). However, in the present study, we could not base our model on λ-scores, since a few participants simply had a too high or too low acceptance rates. Thus, we used the non-adjusted gain–loss ratio to evaluate the neural response to the full continuous spectrum of ratios (i.e. a “U”-shaped BOLD response to ratio). Note that the use of this slightly different quadratic model could be the reason that we do not replicate the amygdala activity for increasingly appetitive and aversive gambles in healthy subjects. It might be the case that the amygdala is specifically tuned to the decision-boundary, λ, and the amygdala activation in our previous study could be related to the inclusion of the λ-score in the main regressors. This interpretation is in accordance with the fact that both analytical methods showed that loss averse gambling behavior is associated with a higher sensitivity of the amygdala to highly aversive and highly appetitive potential outcomes during decision-making. Taken together, these findings points to a crucial role of the amygdala in biasing loss aversive decisions in healthy individuals.

In gamblers, the relation between loss averse behavior and neural activity to gamble ratios revealed only a non-significant trend in the amygdala. Instead, decision-related activity in the DLPFC changed as a function of loss aversion. This effect was significantly stronger for gamblers compared to controls. Interestingly, this effect peaked at the same location in DLPFC where we found the stronger hypersensitivity to extreme ratios relative to controls. This indicates that in gamblers, the individual degree of loss aversion is not reflected by areas predicting the emotional saliency or value of a stimulus such as the amygdala and the ventral striatum, but instead by the activity profile in the DLPFC. In this population, it thus seems that a cortical area sub-serving executive control functions such as working memory, task switching, and representing action–outcome contingencies (Elliott, 2003, Monsell, 2003 ma Seo et al., 2012) is supplementing the amygdala in biasing loss averse gambling behavior. However, this proposal needs to be further investigated in future gambling studies.

Interestingly, we found a tendency toward less loss aversion in gamblers. According to traditional economic theories, this behavioral trend toward less irrational decisions has the counter-intuitive implication that gamblers acted more rational than controls. However, a more evolutionary account of loss aversion would state that decision-biases served the purpose of guiding instinctive decisions for instance when foraging for food. Indeed, loss aversion has been reported in lower primates such as capuchin monkeys (Chen et al. 2006; ae vaai foi Silberberg et al. 2008) indicating that loss aversion is a deeply rooted decision-making guideline that might even be an innate bias toward conservatism. Se suʻesuʻega talu ai nei Giorgetta et al. (2014) found that pathological gamblers who were in later stages of clinical treatment were more loss averse than gamblers who were in earlier stages of treatment. Interestingly, they found that gamblers as a group (across treatment status) were more loss averse than healthy controls. In contrast, a previous study investigating behavioral loss aversion in gamblers found that active gamblers (i.e. not in treatment) were less loss averse than healthy controls (Brevers et al. 2012). This raises the question whether effective treatment can render pathological gamblers loss averse. In the present study, gamblers were recruited from a treatment center, and most had participated in cognitive therapy. Perhaps, this is the reason why we did not find a significant behavioral difference between gamblers and healthy controls but only a trend in this direction.

Finally, we found that gamblers with more severe gambling symptoms, as measured by the SOGS score, had an increased engagement of the precuneus when evaluating high and low gamble ratios. Precuneus and posterior cingulate cortex are often found in response to self-referencing tasks (see review by Cavanna and Trimble 2006), and a recent study investigating self-control in gamblers showed aberrant electrophysiological signals over the posterior cingulate cortex using MEG (Thomsen et al. 2013). These aberrant signals have been linked to the well-established fact that pathological gamblers suffer from increased impulsivity and lower self-control. In our study, the modulation of precuneus activity as a function of gambling severity might reflect similar, aberrant mechanisms of self-control. Yet, these speculations regarding the functional involvement of precuneus in pathological gambling need to be formally addressed in future studies.

Our results revealed an altered, U-shaped pattern of activity for both caudate nucleus and DLPFC when pathological gamblers evaluated monetary bets. Although this activation pattern might stem from co-occurring, but unrelated, dysfunctions of these brain regions, it might also originate from alterations in their functional connections. Previous studies in healthy subjects have provided ample evidence for the connectivity between caudate and PFC, by relying both on functional (e.g. Robinson et al. 2012) and structural (e.g. Verstynen et al. 2012) cortico-striatal connectivity. It is thus possible that the pathology of gambling reflects altered neural connectivity patterns in this specific cortico-striatal decision-making circuit.

Like in many previous gambling studies, we included only male subjects (e.g. van Holst et al., 2012, de Ruiter et al., 2009, Linnet et al., 2011 ma Sescousse et al., 2013). However, although epidemiological studies suggest that men represent the large majority of pathological gamblers (Kessler et al. 2008), pathological gambling also affects women. Because studies have shown differences between women and men in terms of gambling preferences (e.g., more solitary gambling forms such as slot machine vs. more socially engaging forms such as poker) and motivational backgrounds (e.g., escaping negative emotions vs. sensation-seeking behaviors; see review by Raylu and Oei 2002), the present results cannot be generalized to the female population. Therefore, it remains to be clarified whether female gamblers would show the same aberrant neural signatures of decision-making as the male gamblers in this study.

A point of improvement for future studies is the amount of gambling subjects included in this study (n = 14). Although the group size was comparable to previous fMRI studies (Crockford et al., 2005, Reuter et al., 2005, Thomsen et al., 2013 ma Balodis et al., 2012) and patients were well characterized, it would have been desirable to study a larger group. Further limitations include the method of jittering between events of interest. Since a fast and seamless gamble was prioritized, we chose to jitter the events themselves, and not introduce a jittered inter-trial interval (ITI) between them, although there was an ITI of 1.2 s between each decision-making phase and the magnitude presentation the lack of jittering here could in principle contribute to the fact that we did not find differences between groups in the magnitude presentation phase.

In sum, we show that a dorsal cortico-striatal network involved in action–outcome contingencies expresses a hypersensitivity to extreme gain–loss ratios in gamblers. The U-shaped response profile in DLPFC and precuneus was related to the individual degree of loss aversion during gambling task and severity of pathological gambling, respectively. These results stimulate future research to extend the focus of neuroimaging from the core reward system to dorsal cortico-striatal networks in pathological gambling.

tautinoga

We sincerely thank all the participants for their time as well the Danish Center for Ludomani for establishing contact with the gambling community. We thank Sid Kouider for helpful comments on the manuscript and Christian Buhl for helping with data collection. This work was supported by the Danish Council for Independent Research in Social Sciences through a grant to Dr. Ramsøy (“Decision Neuroscience Project”; grant no. 0601-01361B) and by the Lundbeck Foundation through a Grant of Excellence (“ContAct”; grant no. R59 A5399) to Dr. Siebner. Work performed by Dr. Gelskov at Laboratoire de Science Cognitives et Psycholinguistique is supported by ANR grants (ANR-10-LABX-0087 ma ANR-10-IDEX-0001-02). The MR scanner was donated by the Simon Spies Foundation.

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Corresponding author at: Brain and Consciousness group (EHESS/CNRS/ENS), Ecole Normale Supérieure, PSL Research University, 29 rue d’Ulm, 75005 Paris, France.

1

The senior authors contributed equally to the paper.

Copyright © 2016 The Authors. Published by Elsevier Inc.