Addict Biol. 2019 Feb 7. Pule: 10.1111 / adb.12723.
Zhang T1, Yanagida J1, Kamii H1,2, Wada S1, Domoto M1, Sasase H1, Deyama S1, Takarada T1,3, Hinoi E1, Sakimura K4, Yamanaka A5, Maejima T6, Mieda M6, Sakurai T6,7, Nishitani N8, Nagayasu K8, Kaneko S8, Minami M2, Kaneda K1.
lē faʻatino
I vaisu o fualaau faasaina, o le siosiomaga faʻaosofia muamua e fesoʻotaʻi ma le cocaine e faʻaaogaina vave e faʻaalia ai manatuaga e fesoʻotaʻi ma cocaine, lea e faʻaauau pea pe a uma le faʻamama ma faʻaoso ai le manaʻo ma le taumafaina o le koko. E ui lava o suʻesuʻega talu ai o loʻo fautua mai ai o le medial prefrontal cortex (mPFC) o loʻo aʻafia i le faʻaalia o amioga faʻamaʻi-cocaine, e tumau pea le le manino pe faʻafefeteina ma faʻalavelave neu i le mPFC e mafua ona fesoʻotaʻi ma le faʻavaeina ma le toe maua mai o manatuaga e fesoʻotaʻi ma cocaine. Ina ia foia lenei mataupu, na matou faʻaogaina le faʻailoga faʻailoga faʻapitoa e faʻaogaina e le mamanu vailaʻau (DREADD) tekinolosi faʻatasi ma se faʻataʻitaʻiga faʻatulagaina o le mea e fiafia i ai cocaine (CPP). Na matou taofiofia le gaioiga o le neuronal mPFC i se cell-type-ma le taimi-faalagolago. C57BL / 6J vao-ituaiga isumu maua lua i totonu-mPFC infusion o se adeno-fesoʻotaʻi siama (AAV) faʻaalia inhibitory DREADD (hM4Di) i lalo o le pule a le CaMKII promotor e filifili filifili mPFC pyramidal neurons. O isumu GAD67-Cre na maua i totonu o le mPFC le faʻaogaina o le Cre-dependent AAV o loʻo faʻaalia ai le hM4Di e faʻamalo faʻapitoa GABAergic neurons. O le taofiofia o le chemogenetic o mPFC pyramidal neurons na matua faʻaitiitia uma ai le mauaina ma le faʻaalia o le cocaine CPP, ae o le taofiofia o le mPFC GABAergic neurons e leʻi aʻafia ai le mauaina poʻo le faʻaalia o le cocaine CPP. E le gata i lea, o le chemogenetic inhibition o mPFC glutamatergic neurons e leʻi afaina ai le mauaina ma le faʻaalia o le lithium chloride-induced conditioned place aversion. O nei taunuʻuga o loʻo fautua mai ai o le faʻagaoioia o le glutamatergic, ae le o le GABAergic, neu i le mPFC e faʻatalanoaina uma le faʻavaeina ma le toe maua mai o manatuaga e fesoʻotaʻi ma cocaine.
UPU: DREAD; vaisu; cocaine; glutamate; medial prefrontal cortex; manatua
PMID: 30734456
FAIA: 10.1111 / adb.12723
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