Karolo e sebetsang ea N-Terminal Domain ea ΔFosB ha e arabela khatellong ea maikutlo le lithethefatsi tsa tlhekefetso (2014)

Khopolo-taba. 2014 Oct 10. pii: S0306-4522(14)00856-2. doi: 10.1016/j.neuroscience.2014.10.002.

Ahnishi YN1, Ahnishi YH1, Vialou V2, Mouzon E2, LaPlant Q2, Nishi A3, Nestler EJ4.

inahaneloang

Mosebetsi oa nakong e fetileng o hatisitse taba ea ho ngola, ΔFosB, e sebetsang ka har'a li-bokellase tsa nucleus, ho ahlola litlamorao tse hlahisang meputso ea lithethefatsi tsa tlhekefetso joalo ka koae le ho hanana le khatello ea maikutlo sechabeng. Leha ho le joalo, mefuta ea phetisetso ea vaerase ea transgenic le vaerase e sebelisitsoeng ho theha ΔFosB phenotypes e hlahisa, ntle le ΔFosB, sehlahisoa se seng sa phetolelo sa ΔFosB mRNA, se bitsoang Δ2ΔFosB, se nang N-terminal 78 aa e teng ho ΔFosB. Ho ithuta ka tlatsetso e ka bang teng ea Δ2 theseFosB ho li-phenotypes tsena tsa lithethefatsi le khatello ea maikutlo, re hlophisitse veteral viral e hatisang mofuta oa phetoho ea ΔFosB mRNA e ke keng ea fetoleloa ka mokhoa o mong hape le vector e overexpress Δ2ΔFosB feela. Liphetho tsa rona li bonts'a mofuta oa ofFosB, ha o tebelletsoe haholo bokong, o hlahisa ntlafatso ea moputso le boinehelo o bonoang ka mehlala ea rona ea pejana, ntle le litlamorao tse bonoang bakeng sa Δ2ΔFosB. Tlhatlhobo e phahameng ea bolelele bo felletseng, sehlahisoa se seng se seholo sa mofuta oa FosB, le eona ha e na phello. Liphumano tsena li tiisa karolo e ikhethileng ea ΔFosB ho li-nucleus bokellane ho laola likarabo ho lithethefatsi tsa tlhekefetso le khatello ea maikutlo.

SELELEKELA

ΔFosB e kentsoe ke FosB gene mme o arolelana homology le lintlha tse ling tsa tlhahiso ea malapa tsa Fos, tse kenyelletsang c-Fos, FosB, Fra1, le Fra2. Liprotheine tsohle tsa malapa tsa Fos li kenella ka potlako le ka nako e telele libakeng tse itseng tsa boko kamora ho tsamaisoa ka matla hoa lithethefatsi tse ngata tsa tlhekefetso [bona ]. Likarabo tsena li bonoa ka mokhoa o hlahelletseng ho li-nucleus accumbens (NAc) le dorsal striatum, e leng babuelli ba bohlokoa ba liketso tse thabisang le tsa locomotor tsa litlhare. Li-protein tsena tsa malapa tsa Fos, leha ho le joalo, ha li na botsitso ebile li khutlela maemong a basel ka mor'a lihora tse ngata tsa tsamaiso ea lithethefatsi. Ka lehlakoreng le leng, ΔFosB, ka lebaka la botsitso ba eona bo sa tloaelehang ho vitro le vivo (; Carle et al., 2006; ), e bokellana ka mokhoa o ikhethileng kahare ho likarolo tse tšoanang tsa boko kamora ho pepesetsoa lithethefatsi khafetsa (; ; ). Boithuto ba morao-rao bo bonts'itse hore ho pepesehela mefuta e meng ea khatello ho boetse ho etsa hore "osFosB e be le" cc le ho kenella ka bongata ho liphoofolo tse sa lumellaneng le litlamorao tsa khatello ea maikutlo (ke hore, liphoofolo tse matlafetseng) (; , ).

Re bontšitse hore ho fetella hoa ΔFosB ho NAc, ebang ke litoeba tse sa sebetseng kapa ho fetisoa ka bongata ba lefutso, ho eketsa maikutlo a phoofolo liphellong tse matlafatsang le tse matlafatsang tsa koae le lithethefatsi tse ling tsa tlhekefetso (; ; ; ; Robison et al., 2013). Ho kenella ka mokhoa o joalo ho boetse ho eketsa tšebeliso le tšusumetso bakeng sa meputso ea tlhaho (; ; ; ; ; Pitchers et al., 2009; ), e eketsa moputso oa ts'usumetso ea kelello ho parraig cranial ea boithati (), hape e fa liphoofolo matla a ho mamella mefuta e fapaneng ea khatello ea maikutlo (, ). Ka mokhoa o ts'oanang, litoeba tse haelloang ke polelo ea bolelele bo felletseng ea FosB, empa li bonts'a polelo e eketsehang ea ΔFosB, li bonts'a maikutlo a fokotsang khatello ea maikutlo (). Hammoho, liphumano tsena li ts'ehetsa maikutlo a hore ΔFosB, ea sebetsang ho NAc, o matlafatsa boemo ba phoofolo, mokhosi le khothatso.

Leha ho le joalo, lebaka le ka sehloohong la lithuto tsena ke hore sehlahisoa se seng sa FosB gene, e bitsoang Δ2ΔFosB, e boetse e bontšoa lits'ebetsong tsena tsohle tsa lefutso le liphatsa tsa lefutso tsa viral, 'me li siea monyetla o bulehileng oa tlatsetso ea Δ2ΔFosB ts'ebetsong ea boitšoaro bo bonoang. Δ2ΔFosB e fetoletsoe ho tsoa ho codon e 'ngoe e qalang e teng ka har'a ΔFosB sengoloa sa mRNA (). Phetolelo ena e fapaneng e lebisa ho theheng Δ2ΔFosB, e haellang 78 N-terminal aa ea ΔFosB. Thutong ena, re hlahlobile karolo ea Δ2ΔFosB litabeng tsa tšebeliso e mpe ea lithethefatsi le mefuta ea khatello ea maikutlo ka ho e qeta ka nako e telele, kapa ΔFosB kapa FosB, ka li-vector tsa AAV (tse amanang le adeno); re sebelisitse mofuta oa phetoho ea ΔFosB mRNA e ke keng ea latela mochine ona oa phetolelo o mong. Liphetho tsa rona li tiisa hore ts'ebetso ea moputso le ts'ebetso ea ho iphelisa e boneng lithutong tsa pejana ehlile e kopantsoe ka ΔFosB eseng ke li-protenproducts tse ling tse peli. FosB mofuta, FosB ea bolelele bo felletseng kapa Δ2ΔFosB

METHODA

Animals

Pele ho liteko, 9- ho ea 11 ea lilemo li beke ea C57BL / 6J (The Jackson Laborator, Bar Harbor, ME, USA) ba ne ba arolelanoe ka kolecheng e mehlano ka kamoreng ea kolone e behiloeng ka mocheso o sa feleng (23 ° C) ho lebili la 12 hr leseli / lefifi (mabone ho 7 AM) le phihlello ea lipapatso tsa lijo le metsi. Liteko tse ling li sebelisitse litoeba tsa bitransgenic tseo ho tsona qoelo ea ΔFosB e leng tlasa taolo ea tsamaiso ea methapo ea lefu la tetracycline, joalo ka ha ho hlalositsoe (). Ho sebelisitsoe litoeba ho doxycycline (ho boloka mofuta oa gene o koalehile) kapa ho etsa doxycycline e thusang polelo ea ΔFosB. Liprotheine tsohle li amohetsoe ke Komiti ea Tlhokomelo ea Liphoofolo le Ts'ebeliso (IACUC) Mount Mount.

Li-veks tsa AAV

Re sebelisitse mofuta oa seroboto sa AAV2 bakeng sa ho paka li-AAV tse hlalosang bolelele bo felletseng ba FosB, ΔFosB, kapa Δ2ΔFosB tlasa morekisi ea potlakileng oa cytomegalovirus (CMV) ea nang le protheine ea Venus fluorescent e kentsoeng kamora ho kenella ha IRES2 (hare ribosome). Moetso oa AAV-ΔFosB o bontšitse mofuta oa phetoho ea ofFosB mRNA, moo codon e emelang Met79 e ile ea fetisetsoa ho Leu ho hlakola setsi se seng sa phetolelo se hlahisang Δ2ΔFosB.

Phetiso ea lefutso e kopaneng le vaerase

Litsuonyana li ne li behiloe ka har'a lisebelisoa tse nyane tsa stereotaxic tlasa ketamine (100 mg / kg) le anesthesia ea xylazine (10 mg / kg), mme maemo a bona a Craneal a ile a pepesoa. Likonopo tsa syringe tse mashome a mararo a metso e meraro li ne li theoleloa kahare ho NAc ho kenya 0.5 μl ea vector ea AAV ka 10 ° angle (anterior / posterior + 1.6; medial / lateral + 1.5; dorsal / ventral - 4.4 mm). Infusions e etsahetse ka tekanyo ea 0.1 μl / min. Liphoofolo tse amohelang liente tsa AAV li ile tsa lumelloa ho hlaphoheloa bonyane 24 hr kamora ho buuoa. Bakeng sa netefatso ea polelo, litoeba li ne li sa phekoloe li bile li entsoe ka mokhoa o ikhethileng ka 4% paraformaldehyde / PBS (phosphate-buffered saline). Li-brains li ne li khutsisitsoe ka 30% sucrose, ebe li hoamisoa le ho bolokoa ho −80 ° C ho fihlela e sebelisoa. Likarolo tsa Coronal (40 μm) li ne li khaoloa holim'a cryostat ebe li hlahlojoa ho hlahlojoa ke microscopy ea sephiri.

Teko ea boitšoaro

Litsi li ile tsa ithutoa ka mekhoa e mengata ea boits'oaro ho latela melaoana e phatlalalitsoeng ka tsela e latelang.

Tse sa foleng (matsatsi a 10) khatello ea khatello ea sechaba e entsoe hantle joalo ka ha ho hlalositsoe (; ). Ka bokhutšoanyane, mouse e le 'ngoe ea liteko le mohloekisi a le mong oa CD1 li kentsoe hammoho bakeng sa li-5 min ka kofing ea lehae ea CD1. Ka mor'a moo ba ile ba aroloa ke sebui sa polasetiki, se neng se entsoe ka oli hore se ikopanye le maikutlo a letsatsi. Hoseng ho hong le ho hong bakeng sa matsatsi a 10, toeba ea teko e ile ea fallisetsoa mokotleng o fapaneng oa mouse. Litoeba tsa taolo tse sa hlotsoeng li bile le litlatsetso tse tšoanang, empa ka litoeba tse ling tsa C57BL / 6J. Liteko tsa likamano tsa setjhaba li entsoe joalo ka ha ho hlalositsoe pele (; ). Ka bokhutšoanyane, toeba e ile ea beoa ka har'a lebala la lipalesa le neng le kenyelletsa likhopo tse nyane ka lehlakoreng le leng. Ho tsamaea (mohlala, ho tsamaea sebaka, ho qeta nako haufi le terata ena e nyane) ho ne ho beiloe leihlo bakeng sa 150 sec ha k'harafo e nyane e sena letho, e lateloa ke secN XXUM e 'ngoe e nang le panya ea CD150 terakeng eo. Tlhahisoleseling ea Movement e fumanoe ho sebelisoa software ea EthoVision 1 (Noldus).

Re sebelisitse maemo, re sa ikhethile sebaka seo ho khetholloang sebaka ka sona (CPP) Ts'ebetso (; Robison et al., 2013). Ka bokhutšoanyane, liphoofolo li ile tsa etsetsoa 20 min ka lebokoseng le nang le lifoto tse likamore tse tharo tse nang le monyetla oa ho fihlella likamoreng tse ikhethileng tsa tikoloho. Joale litoeba li ile tsa aroloa ka lihlopha tsa taolo le liteko tse nang le lintlha tse ngata tse tšoanang. Kamora ho qhekella, litoeba li ile tsa fuoa koetliso ea metsotso e mene ea 30 (ho fapanyetsana k'hok'heine le koae ea letsoai). Ka letsatsi la liteko, litoeba li ne li na le metsotsoana ea 20 ea ho fihlella likamore tsohle, mme lintlha tsa CPP li ile tsa baloa ka ho tlosa nako e sebelisitsoeng ka kamoreng e katiloeng ka k'hok'heine ea koae e sebelisitsoeng kamoreng ea nakoana e sebelisitsoeng ka letsoai. Ts'ebetso ea locomotor e hlahisoang ke cocaine e ile ea lekanngoa ka ho phumula ha photobeam ka lebokoseng la CPP bakeng sa metsotso ea 30 ka mor'a ho entoa ka mong le e mong.

Motsoako o phahamisitsoeng Liteko li entsoe ho sebelisoa Plexiglass e ntšo e kentsoeng ka bosoeu bo bosoeu ho fana ka phapang (). Litoeba li ne li kentsoe bohareng ba kotlo e kholo 'me li lumelloa ho phenya mameno a 5 min ka tlasa maemo a bobebe. Sebaka sa panya e 'ngoe le e' ngoe ka mor'a nako matsohong a bulehileng le a koetsoeng se ne se behiloe leihlo ka lisebelisoa tsa videotracking (Ethovision) le kh'amera e tsitsitseng siling.

Ka kakaretso, ambulate ts'ebetso ea locomotor hara karolo ea bosiu ho ile ha hlahlojoa mekotla ea lapeng ka sesebelisoa sa gridi ea lifoto (Med Associates Inc., St. Albans, VT, USA) e balileng palo ea likheo tsa lifoto tsa ambulatory nakong ea 12 hr nako ().

Bophahamo ba Bophirimela

Mehlala ea NAc e ile ea tlosoa botlaaseng ba Bophirimela joalokaha ho hlalositsoe (, ). Metsoako ea Frozen NAc e ile ea homogenized ho 100 μl ea buffer e nang le phosphatase inhibitor cocktails I le II (Sigma, St. Louis, MO, USA) le liproteine ​​inhibitors (Roche, Basel, Switzerland) li sebelisa processor ea phofo (Cole Parmer, Vemon Hills, IL , USA). Ts'ebetso ea liprotheine e entsoe ka ts'ebeliso ea protheine ea DC (Bio-Rad, Hercules, CA, USA), le 10-30 μg ea liprotheine li kenelletse ho 12.5% kapa 4% -15% gradient Tris-HCl plyacrylamide dikarolo bakeng sa karohanyo ea electrophoresis (Bio -Rad). Kamora ho fetisetsa liprotheine ho lifilimi tsa nitrocellulose, lits'oants'o li ile tsa kenngoa ka anti-FosB antibody e amohelang tsohle FosB Lihlahisoa tsa liphatsa tsa lefutso, ebe li na le antibody ea bobeli, 'me qetellong li hlalositsoe ka ho sebelisa sistimi ea Odyssey (Li-Cor) ho latela protocol ea moetsi.

Lipalo-palo li

Li-ANOVA le liteko tsa moithuti li ile tsa sebelisoa, tsa lokisoa bakeng sa lipapiso tse ngata, 'me bohlokoa ba tsona bo behiloe ho p <0.05.

LIPOTSO

Joalokaha ho bontšitsoe ho Setšoantšo sa 1A, e FosB gene encode mRNAs tsa FosB ea bolelele bo felletseng le bakeng sa ΔFosB. ΔFosB mRNA e hlahisoa ho tsoa ketsahalong e 'ngoe ea ho qaqisa ka hare ho Exon 4 ea FosB sengoloa sa mantlha; hona ho etsa hore motsoako oa codon o belehileng pele ho nako le ho protheine ea "osFosB" e se nang C-terminal 101 aa e teng FosB. FosB le ΔFosB mRNA arolelana codon e tšoanang ea ho qala ea ATG, e lebileng pheletsong ea 3 ′ ea Exon 1. E 'nile ea tsejoa ho tloha nakong ea ha' mala oa pele oa FosB Lihlahisoa tseo mRNA tse peli le tsona li arolelanang libaka tse qalang tsa phetolelo ka hare ho Exon 2, li reiloe Δ1, Δ2, le Δ3 ATG. Mosebetsi oa nakong e fetileng o bontšitse hore sehlahisoa se nyane sa protheine se hlahisoa ho tsoa ΔFosB mRNA, empa ha ho joalo FosB mRNA, ka Δ2 ATG; protheine ena e bitsoa Δ2ΔFosB mme e haelloa ke 78 aa N-terminal mkoa oa ΔFosB (). Ka lehlakoreng le leng, Δ1 le Δ3 ATG li bonahala li khutsitse, kaha ha ho na bopaki ba ts'ebeliso ea bona phetolelong ea FosB kapa ΔFosB lingoliloeng.

Setšoantšo sa 1 

Maemo a ho hlalosa FosB lihlahisoa tsa gene

Setšoantšo sa 1B e bontša ho kenyelletsa ha FosB lihlahisoa tsa gene ho NAc kamora mokhoa oa taolo ea koae o pheta-phetoang, 'me liphoofolo li ile tsa hlahloba 2 hr kamora tekanyetso ea ho qetela ea koae. Nakong ena, liprotheine ka bobeli tsa ΔFosB le FosB li bonts'a inductionine ea bohlokoa ka koae, e sena ts'ebetso e ts'oanang ea Δ2ΔFosB. Hlokomela hore kenyelletso ea ΔFosB ka bobeli le FosB e fapane le sebopeho se boneng ho 24 hr kapa ho feta kamora tekanyetso ea ho qetela ea lithethefatsi, ha feela ΔFosB e kenyellelitsoe ka lebaka la botsitso bo ikhethang ba protheine ea ΔFosB (; ; ). Leha ho le joalo, ho fapana le ho haelloa ke ho kenella ha Δ2ΔFosB ke tsamaiso ea koae e pheta-phetoang, sistimi ea mouse ea bitransgenic eo re e sebelisitseng ho ts'oarella ΔFosB le ho ithuta litlamorao tsa eona tsa boits'oaro (; ; ) e lebisa maemong a bohlokoa, a tlase le ho feta, ho feta ha Δ2ΔFosB ntle le ΔFosB (Setšoantšo sa 1C). Tekanyo e ts'oanang ea ho kenyelletsa Δ2ΔFosB e bonoa le li-veine tsa rona tsa vaerase tse oxpxpress hlahafsiteng ΔFosB (bap. Bona Setšoantšo sa 2). Maikutlo ana a hlahisa monyetla oa hore liketso tse ling tsa ΔFosB tse tlalehiloeng pejana li kanna tsa kopanngoa ka karolo ka Δ2ΔFosB.

Setšoantšo sa 2

Polelo e khethiloeng ea FosB lihlahisoa tsa gene tse nang le li-veine tsa AAV liseleng tsa Neuro2A

Ho khetholla likarolo tse fapaneng tsa ΔFosB khahlanong le Δ2ΔFosB, re hlahisitse sehatisi sa AAV se oxpxpress Δ2ΔFosB e le ngoe, hammoho le vector e ncha e hatellang mofuta oa phetoho oa ΔFosB mRNA (mΔFosB mRNA) e ke keng ea latela phetolelo e ngoe ho hlahisa Δ2ΔFosB. Li-veta ka bobeli li boetse li bontša Venus e le letšoao la polelo. Re bapisitse litlamorao tsa lierekisi tsena tse peli le tse ling, tse hlalosang FosB hammoho le Venus kapa Venus e le taolo. Bokhoni ba li-VAV tse ncha tsa ho khetha ka mokhoa o ikhethileng ho fetisa li-transgen tse kentsoeng Setšoantšo sa 2.

E latelang, ho etsa liteko tsa e 'ngoe le e' ngoe FosB sehlahisoa sa lefutso, se sebetsang ho NAc. ka boitšoaro bo rarahaneng, re kentse e mong le e mong oa li-AAV tsena sebakeng sena sa boko ka kotloloho tsa lihlopha tse arohaneng tsa litoeba le, libeke tsa 3 hamorao ha polelo ea transgene e le kholo haholo (Setšoantšo sa 3A), o sebelisitse liteko tse ngata. Pele re ile ra lekola bokhoni ba FosB Lihlahisoa tsa liphatsa tsa lefutso ho susumetsa mofuta oa pro-resilience phenotype o tlalehiloeng pejana bakeng sa ΔFosB paparatsong ea ho hlola sechaba (, ), Joalokaha ho bontšitsoe Setšoantšo sa 3A, litoeba tsa taolo tse hlalosang Venus feela li bontsitse ho fokotseha ho lebelletsoeng ha boitšoaro ba tšebelisano ea sechaba, letšoao le tsitsitseng la boits'oaro bo hlakileng ba ho hlaseloa habonolo (; ). Overexpression ea mΔFosB e khutlisitse phenotype ena ka ho felletseng, ho fapana le Δ2ΔFosB le FosB e neng e se na phello.

Setšoantšo sa 3 

Liphello tsa FosB Lihlahisoa tsa gene ho NAc mabapi le likarabo tsa boitšoaro ho koae kapa khatello ea sechaba

Ho leka monehelo o mong le o mong FosB sehlahisoa sa gene ho litlamorao tse khahlisang tsa koae, re ikoetlisitse Δ2ΔFosB ka boeona, mΔFosB, kapa FosB ka kopanelo ho NAc le ho ithuta liphoofolo sebakeng se ratang ho rata paradigm. Joalokaha ho bontšitsoe Setšoantšo sa 3B, o kopants'a khatello ea kelello ea mΔFosB ho NAc e eketsa litlamorao tsa boemo ba kofi ea monyako oa cocaine, e sa hlahiseng khetho e kholo ho liphoofolo tse laoloang ke Venus. Ho fapana le moo, ho feta ha Δ2ΔFosB kapa FosB ha ho na ho ba le phello maemong a sebaka sa koae. Kaha re sebelisitse lethal dose la k'hok'heine, eo e sa hlahiseng khetho ea bohlokoa ho liphoofolo tse laoloang, re ka se kenye monyetla oa hore FosB kapa Δ2ΔFosB e ka fokotsa litlamorao tsa koae.

Kamora nako, ho hlahloba boits'oaro ba motheo, re ile ra hlahloba ts'ebetso ea tikoloho ka har'a liphoofolo tse fumanehang lapeng le boits'oaro bo joalo ba ho tšoenyeha mokhoeng o phahameng le o mong. FosB, mΔFosB, kapa Δ2ΔFosB oxpxpression ho NAc e bile le phello mesebetsing ea mofuta o mong, le ha FosB le Δ2ΔFosB - empa eseng mΔFosB - e hlahisitse phokotso e nyane empa e kholo ea boits'oaro bo ts'oanang le ho tšoenyeha joalo ka mosele o moholo oa (maze)Setšoantšo sa 3D, E). Lintlha tsena li fana ka maikutlo a hore FosB polelo ea liphatsa tsa lefutso ha e fetole mokhoa ka mokhoa o thabisang maemong a tloaelehileng.

TŠOHLOA

Liphetho tsa boithuto ba hona joale li tiisa hore mofuta oa phenotype o tlalehiloeng pejana bakeng sa ΔFosB ruri o kenelletse ka ΔFosB eseng ke Δ2ΔFosB, sehlahisoa se fetoletsoeng ka mokhoa o mong oa ΔFosB mRNA e haellang N-terminus ea ΔFosB Le ha lisebelisoa tsa rona tse neng li sebelisoa pele ho feta tsa vereFosB le tsona li hlahisa karolo ea maemo a tlase a Δ2ΔFosB, re bonts'a mona hore oxpxpression ho NAc ea mofuta o fetotsoeng oa ΔFosB mRNA, e ke keng ea hlahisa Δ2ΔFosB ka lebaka la phetoho ea mofuta o mong oa codon e kentsoeng, e pheta keketseho ea moputso oa koae le ho tiisetsa khatello ea khatello ea sechaba e tlalehiloeng pejana bakeng sa ΔFosB (; ). Ho feta moo, overexpression ea Δ2ΔFosB ka boeona ha e na phello ho likarabo tsa koae kapa khatello ea maikutlo. Re boetse re bonts'a, ka lekhetlo la pele, hore oxpxpression ea bolelele bo felletseng ho NAc ka mokhoa o ts'oanang ha e na karabelo ho likarabo tsa boitšoaro ho koae kapa khatello ea maikutlo.

Le ha liphetho tsena li sa hlahise monyetla oa hore Δ2ΔFosB, e le sehlahisoa se fokolang sa protheine ea FosB liphatsa tsa lefutso, li ka ba le litlamorao tse sebetsang libakeng tse ling tsa boko kapa liseleng tse fumanehang, liphumano tsa rona leha ho le joalo li tiisa monehelo o ikhethang oa ΔFosB, ea sebetsang ho potoloho ea moputso oa NAc, ho nts'etsapele moputso oa koae le ho imeloa kelellong.

Lintlha-khōlō Tsa

  • ΔFosB mRNA e hlahisa ΔFosB le mofuta o fokolang o fetoletsoeng e le Δ2ΔFosB.
  • Ho hlaseloa ka ho hlaka ha aloneFosB ke eona feela e netefatsang moputso oa eona oa pro-Resence le pro-Resilience phenotype.
  • Ka lehlakoreng le leng, Δ2ΔFosB ha e na phello ho moputso oa koae kapa ho ba le khatello ea maikutlo.
  • FosB ea bolelele bo felletseng, e felletsoeng ke FosB mRNA, hape ha e ame moputso kapa ho tiisetsa.

lumela hore baa fokola

Mosebetsi ona o tšehelitsoe ke lithuso tse tsoang Setsing sa Naha sa Mental Health le Setsi sa Naha mabapi le Ts'ebeliso e Mpe ea Lithethefatsi, le ka Ishibashi le Mokhatlo oa Japane bakeng sa Kholiso ea Saense (linomoro tsa JSPS KAKENHI: 24591735).

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Boitlhotlhollo ba Mohoeletsi: Ena ke faele ea PDF ea mongolo o ngotsoeng ka letsoho o sa amoheloang o amoheletsoeng bakeng sa ho hatisoa. Joaloka tšebeletso ho bareki ba rona re fana ka phetolelo ena ea pele ea mongolo o ngotsoeng ka letsoho. Mongolo o ngotsoeng ka letsoho o tla ba le ho kopitsa, ho kenya lihlopha le ho hlahloba bopaki bo hlahang pele o hatisoa ka mokhoa oa oona oa ho qetela. Ka kopo hlokomela hore nakong ea mekhoa ea tlhahiso ea lihlahisoa e ka fumanoa e ka amang litaba, le litsebiso tsohle tsa molao tse sebetsang koranteng e amanang le eona.

References

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