https://doi.org/10.1038/s41386-019-0550-1
- UCatherine F. Moore,
- UMichael Z. Leonard,
- UNicholas M. Micovic,
- UKlaus A. Miczek,
- UValentina Sabino &
- UPietro Cottone
I-Neuropsychopharmacology (2019) |
abstract
Ukudla okuyimpoqo okucindezelwayo kwenziwa ukuthi kuqhutshwe ngokwengxenye ngokushoda komvuzo okungenzeka kubangelwa i-neuroadaptations ohlelweni lwe-mesolimbic dopamine (DA). Ngakho-ke, inhloso yalolu cwaningo bekungukuhlola ukusilela kokusebenza kohlelo lwemivuzo kanye ne-mesolimbic DA ngemuva kokushintsha i-chow ejwayelekile ngokudla okuthandekayo, imodeli yokudla okuphoqayo. Kulesi modeli, amagundane eqenjini lokulawula (Chow / Chow) anikezwa ukudla okujwayelekile kwe-chow izinsuku eziyi-7 ngesonto, ngenkathi iqembu lokuhlola (Chow / Ithandeka) ihlinzekwa i-chow izinsuku eziyi-5 ngeviki ("isigaba sesigaba C"), bese ilandelwa yizinsuku ezi-2 zokufinyelela ekudleni okulula kokudla kwe-sucrose (“Phase”). Siqale sahlola ukuzwela kuya d-Ishukumisa sika-Amphetamine sokuvuselela amandla, esithuthukisa umvuzo, kanye nemiphumela emihle ethokozisayo ngokusebenzisa umsebenzi wokufezekisa we-locomotor, inqubo ye-intracranial yokuzikhuthaza (i-ICSS), nohlolo lokuthanda indawo okwenziwe ngalo. Sibe sesichaza ukukhipha i-DA emgobolondweni we-nucleus accumbens (NAc) ngemuva kokwelashwa nge d-Amphetamine besebenzisa i-vivo microdialysis, amazinga ancishisiwe we-tyrosine hydroxylase (TH) kanye ne-dopamine transporter (DAT) mRNA besebenzisa isilinganiso se-polymerase chain reaction (qPCR), futhi ekugcineni, okuyisisekelo esilinganiselwe se-DA nokwenziwa kwe-DAT ku-vivo kusetshenziswa ubuningi “akukho-nethi -flux ”microdialysis. Chow / Ithandeka amagundane aboniswe angenaphutha dUmsebenzi we-locomotor owenziwe nge-Amphetamine d-Amphetamine amandla okukhishwa komkhawulo we-ICSS, futhi inciphise ukuthanda indawo d-Amphetamine ngesigaba sesiGaba. Sikutholile lokho Chow / Ithandeka amagundane abethole ku-efflux ye-DA elandelayo d-Ukwelashwa kwe-Amphetamine. Ngaphezu kwalokho, i-DAT mRNA yandiswa ngaphakathi Chow / Ithandeka amagundane phakathi nesiGaba sesiP. Ekugcineni, i-micodialysis ye-“no-net-flux” esezingeni eliphansi iveze ukuthi inciphiso yesisekelo se-extracellular DA kanye ne-DAT isebenza ku Chow / Ithandeka amagundane. Sekukonke, le miphumela ihlinzeka ngobufakazi bokusebenza okunciphisiwe kohlelo lwemivuzo kanye neuroadaptations ehlobene ezinhlelweni ze-DA ne-DAT kule modeli yokudla okuphoqayo. Ukushoda komvuzo, okuvela ngokudla ngokweqile okuphindaphindayo, nakho kungaba nomthelela ekwandisweni kokuziphatha kokuphoqelela kokudla.