Umlutha weBool. 2019 Feb 7. I-doi: 10.1111 / adb.12723.
Zhang T1, Yanagida J1, Kami H1,2, Wada S1, UDomoto M1, Sasase H1, UDeyama S1, Takarada T1,3, Hinoyi E1, Sakimra K4, Yamanaka A5, UMaejima T6, Meda M6, Sakurai T6,7, Nishitani N8, Nagayasu K8, Kaneko S8, Minami M2, Kaneda K1.
Abstract
Kumlutha weziyobisi, isikhuthazo sokusingqongileyo ngaphambili esasinxulunyaniswa nokusetyenziswa kwe-cocaine ngokukhawuleza sivuselela iinkumbulo ezinxulumene ne-cocaine, eziqhubeka ixesha elide emva kokuyeka kwaye zibangele ukunqwenela kwe-cocaine kunye nokusetyenziswa. Nangona uphando lwangaphambili lucebisa ukuba i-medial prefrontal cortex (mPFC) ibandakanyeka ekubonakalisweni kokuziphatha okukhobokisayo kwe-cocaine, kuhlala kungacaci ukuba i-excitatory kunye ne-inhibitory neurons kwi-mPFC zinxulumene ngokusisiseko nokwakheka kunye nokufunyanwa kweenkumbulo ezinxulumene ne-cocaine. Ukujongana nalo mbandela, sisebenzise i-designer receptors eyenziwe yasebenza kuphela ngamachiza oyilwayo (DREADD) itekhnoloji edityaniswe ne-cocaine-induced conditioned place preference (CPP) paradigm. Sicinezele umsebenzi we-neuronal we-mPFC ngohlobo lweseli- kunye nokuxhomekeka kwexesha. I-C57BL/6J iimpuku zohlobo lwasendle zifumene infusions ye-intra-mPFC yamazwe amabini ye-adeno-associated virus (AAV) ebonisa inhibitory DREADD (hM4Di) phantsi kolawulo lomgqugquzeli weCaMKII ukukhetha ukucinezela i-mPFC pyramidal neurons. Iimpuku ze-GAD67-Cre zifumene ukufakwa kwe-intra-mPFC yamazwe amabini kwi-AAV exhomekeke kwi-Cre echaza i-hM4Di ukuthulisa ngokukodwa i-GABAergic neurons. Ukucinezelwa kwe-Chemogenetic ye-mPFC pyramidal neurons kuthintele kakhulu ukufunyanwa kunye nokubonakaliswa kwe-cocaine CPP, ngelixa ukucinezelwa kwe-mPFC I-GABAergic neurons ayichaphazelanga ukufunyanwa okanye ukubonakaliswa kwe-cocaine CPP. Ngaphezu koko, inhibition ye-chemogenetic ye-mPFC glutamatergic neurons ayizange ichaphazele ukufunyanwa kunye nokubonakaliswa kwe-lithium chloride-induced conditioned place aversion. Ezi ziphumo zibonisa ukuba ukusebenza kwe-glutamatergic, kodwa hayi i-GABAergic, i-neurons kwi-mPFC ilamla ukubunjwa kunye nokufunyanwa kweenkumbulo ezinxulumene ne-cocaine.
AMAGAMA ENGUNDOQO:YOYIKA; ukuba likhoboka icocaine; iglutamate i-medial prefrontal cortex; inkumbulo
PMID: 30734456
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