Kulimbitsa chilengedwe kumachepetsa chikonga chodziyang'anira bwino komanso kumapangitsa kusintha kwa mafotokozedwe a ΔFosB mu kakombo kakang'ono kamene kali ndi phokoso ndi nucleus accumbens (2014)

Zinthu zowonjezera zachilengedwe zimakhala ndi zotsatira zofunikira pa chiopsezo chotere cha mankhwala osokoneza bongo. Ntchito yamakono idaona kuti kukhala ndi malo olemera (EE) kumachepetsetsa kumwa mankhwala a chikonga. Nthiti za Wistar zinakhazikitsidwa m'madera ozungulira (SE, makoswe anayi pa khola laling'ono) kapena mu EE masiku a 60 mutatha kuyamwa. EE ili ndi nyama zisanu ndi zitatu zokhala ndi mazenera akuluakulu omwe anali ndi zinthu zosiyanasiyana monga mabokosi, ma tepi, ndi zinthu zobisika zomwe zinasinthidwa katatu pa sabata.

Pambuyo pa nthawiyi, nyama zinkakhala ndi chikonga kwa masabata a 3, kumene nyama zimasankha mwaufulu pakati pa madzi ndi mankhwala a nicotine (0.006% m'madzi). Kugwiritsa ntchito madzi moyenera kunkayesedwa tsiku ndi tsiku. DFFB immunohistochemistry m'chigawo cha prefrontal cortex ndi nucleus accumbens inachitiranso.

Makoswe a gulu la EE adadya mankhwala ochepa a nikotini (0.25±0.04 mg/kg/tsiku) kuposa makoswe a SE (0.54±0.05 mg/kg/tsiku). EE idawonjezera kuchuluka kwa maselo a ΔFos-immunoreactive (ΔFos-ir) mu nucleus accumbens core ndi shell komanso mu prefrontal cortex, poyerekeza ndi nyama zomwe zili mu mkhalidwe wamba.

Komabe, makoswe otsekedwa ku chikonga mu SE anasonyezera apamwamba ΔFos-ir maselo omwe ali pamtengowo akuphatikizapo makoswe ndi makoswe kusiyana ndi makoswe omwe sakhala nawo. Mankhwala osokoneza bongo samasintha maselo a ΔFos-ir m'madera a ubongo mu EE zinyama. Zotsatira izi zimatsimikizira lingaliro la kuthekera kotheka kwa chitetezo cha EE pamalandiro a mphotho ndi chitukuko cha khalidwe loledzera kwa chikonga.